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S-glutathionylation has been reported to regulate activity of various enzymes. Fluorescent bands were developed with 1mL of substrate containing same volume of each Super Signal West Pico Luminol Enhancer solution and Super Signal West Pico Stable Peroxidase solution at room temperature for 5 min. Equal volume of 5% (w/v) sulfosalisialic acid was used to precipitate all proteins from the tissue cytosols. Standard curve for the plot of Log (MW) versus Ve/V0 was determined using Molecular Weight Marker Kit For Gel Filtration Chromatography (MW-GF-200) from Sigma Chemical Company . On the other hand,Cys82 is in direct contact with substrate (3 A° ) and is exposed on the substrate binding site surface. [ Last edited by wenjie on 2011-5-28 at 18:18 ] |
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8814402
至尊木虫 (职业作家)
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3楼2011-05-29 17:05:28
8814402
至尊木虫 (职业作家)
- 翻译EPI: 509
- 应助: 18 (小学生)
- 贵宾: 0.381
- 金币: 12916.1
- 散金: 47
- 红花: 16
- 帖子: 4183
- 在线: 357.8小时
- 虫号: 1184404
- 注册: 2011-01-06
- 专业: 药物学其他科学问题
【答案】应助回帖
★
sltmac(金币+1): 谢谢~~ 2011-05-29 01:03:13
wenjie(金币+5, 翻译EPI+1): 不好，好多都没翻译出 2011-05-29 13:21:33
sltmac(金币+1): 谢谢~~ 2011-05-29 01:03:13
wenjie(金币+5, 翻译EPI+1): 不好，好多都没翻译出 2011-05-29 13:21:33
| S-glutathionylation has been reported to regulate activity of various enzymes. S-glutathionylation已被报道可调节各种各样酶的活性. Fluorescent bands were developed with 1mL of substrate containing same volume of each Super Signal West Pico Luminol Enhancer solution and Super Signal West Pico Stable Peroxidase solution at room temperature for 5 min. 含有相同体积Super Signal West Pico 发光氨强化剂溶液和Super Signal West Pico 稳定过氧化物酶溶液的1mL底物溶液在室温孵育5分钟是荧光谱带显现. Equal volume of 5% (w/v) sulfosalisialic acid was used to precipitate all proteins from the tissue cytosols. 等体积的5% sulfosalisialic 酸用于沉淀来自组织胞质溶胶的蛋白质.Standard curve for the plot of Log (MW) versus Ve/V0 was determined using Molecular Weight Marker Kit For Gel Filtration Chromatography (MW-GF-200) from Sigma Chemical Company . Log (MW)对Ve/V0 作图的标准曲线使用凝胶过滤色谱用分子marker套装测定.On the other hand,Cys82 is in direct contact with substrate (3 A° ) and is exposed on the substrate binding site surface. 另一方面,Cys82直接与底物接触,暴露在底物结合位点表面. |
2楼2011-05-28 21:19:55
