Study of soil microorganism and diversification of PAHs degradative functional gene community is of great instructive value to microbiological bioremediation of PAHs polluted soil. Contents and diverification of soil PAHs degradative functional genes and PAHs degradation featured microorganisms greatly determine isolation of PAHs degradative strains with high efficiencies, and bioremediation of PAHs polluted soils. Recently, molecular biology greatly broadens our knowlege on diversification of soil microorganisms. With molecular biology, we can get detailed information on community structures of soil microorganisms, as well as floristic compositions of microorganisms. Starting from polluted soils, sampled from a coking plant, aboundance and diversification of soil microorganisms were investigated through analysis of physiochemical properties and PAHs contents of polluted soil. Molecular techniques, such as soil DNA isolation, PCR, realtime PCR and T-RFLP were also applicated. Influential factors of aboundance of soil microorganisms and community diversification were discussed. With PAHs polluted deep soil,soil oxygen exchanging rate was improved by shake to investigate whether it can help degradate PAHs from deep soil. Meanwhile, evolution features of aboundance of soil microorganisms as well as dynamic community diverisification structures were investigated.
回复此楼