24小时热门版块排行榜

返回列表

当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖

billowzeng

金虫 (正式写手)

应助: 1 (幼儿园)
金币: 742.6
散金: 1185
红花: 7
帖子: 368
在线: 173小时
虫号: 731012
注册: 2009-03-25
专业: 环境微生物学

[求助] GenBank 提交序列，<200bp片段不予接收？

我向GenBank提交序列，引物扩增出的目的片段为180bp左右，但GenBank说片段太短，不能接收，这该怎么办呢？
“Some or all of the sequence(s) in your file(s) are less than 200 bp.
Unfortunately, we must inform you that we have a policy not to accept
sequences shorter than 200 bp. ”

回复此楼

» 猜你喜欢

1楼 2011-05-23 10:28:31

已阅回复此楼关注TA 给TA发消息送TA红花 TA的回帖

billowzeng

金虫 (正式写手)

应助: 1 (幼儿园)
金币: 742.6
散金: 1185
红花: 7
帖子: 368
在线: 173小时
虫号: 731012
注册: 2009-03-25
专业: 环境微生物学

★ ★ ★
silicare(金币+3): 鼓励反馈交流 2011-05-27 11:51:52

引用回帖:

Originally posted by mirror3895 at 2011-05-24 08:12:41:
我实验室也有人出现过此类问题，但是后来发现是在提交时对序列描述错误，应该是可以提交的，建议再试试，注意要说明是16Sr DNA V3区DGGE切胶回收的片段（是DGGE的吧？）

还是不接收，我的留言是“The band1 to band12 were isolated from DGGE gel of 16S ribosomal V3 RNA gene, partial sequence. The sequences were about 180bp. This the reason the sequences were shorter than 200bp”
他的回复是“
However, this does not resolve the issue.
Some or all of the sequence(s) in your file(s) are less than 200 bp.
Unfortunately, we must inform you that we have a policy not to accept
sequences shorter than 200 bp. We realize that this has short-term
consequences for submitters, but feel that the long-term improvements
in the database will be helpful for all database users.
”
请问你们实验室同学是怎样提交的呢？
先谢谢了。