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dong5705银虫 (初入文坛)
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[求助]
急+急，求助多糖类摘要翻译
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中文摘要 目的：制备不同分子量片段的银耳多糖，对其进行理化性质、化学结构的研究，并筛选出对抗肿瘤药物具有减毒、升高白细胞作用的最佳活性多糖。 方法：正交试验法考察银耳多糖的提取条件，确定提取工艺；酸水解方法制备不同分子量片段的银耳多糖，通过苯酚-硫酸法、间羟基联苯法、Lorry法及BCA法对其进行理化性质的研究，HPLC法确定其糖组成，并采用甲基化、GC-MS研究其中性糖糖苷键连接方式。对正常小鼠及荷瘤鼠采用静脉注射给药，观察不同分子量、不同侧链样品对抗肿瘤药物的减毒作用。 结果：正交试验法得到银耳多糖的最佳提取工艺为50倍量的水进行煎煮，每次三小时共煎煮三次；通过酸水解法得到20万、3万和4000三个不同分子量片段的银耳多糖；其理化性质测定结果为：20万分子量片段中性糖含量约为76.5%, 酸性糖含量约为19.8%，蛋白含量约为9%；3万分子量片段中性糖含量约为78.2%，酸性糖含量约为22.1%，蛋白含量约为6.2%；4000分子量片段中性糖含量约为83.1%，酸性糖含量约为22.3%，蛋白含量约为8.2%。HPLC法分析结果表明，三个不同分子量片段的银耳多糖均由Man、Glu、Xyl和GlcA组成。GC-MS结果可判定不同分子量片段的银耳多糖均以1→3连接的Man为主链，并含有少量的1→4连接的Xyl；通过对不同分子量片段的银耳多糖构效关系及其药理作用的的研究可知分子量为3万的银耳多糖片段对抗肿瘤药物致外周血白细胞减少具有较好的升高白细胞的作用， 结论：本实验通过对银耳多糖的提取、理化性质、构效关系的研究并结合药理实验，筛选出具有对抗肿瘤药物减毒升白作用的银耳多糖片段，不仅对银耳多糖构效关系的总结及作用机理有明显的推动作用，也对药物开发具有重要意义。 关键词：银耳多糖；理化性质；PMP衍生化；HPLC；GC-MS |
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zuodg
铁杆木虫 (正式写手)
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【答案】应助回帖
dong5705(金币+50, 翻译EPI+1): 谢谢 2011-04-28 22:06:48
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Abstract Purpose: Prepare tremella polysaccharide of different molecule weight, investigate their physiochemical and structural properties and screen polysaccharide of optimum activity which decreases toxicity of antitumor drugs and promotes function of leukocytes. Method: Extraction technology was obtained through orthogonal experiment of tremella polysaccharide. Tremella polysaccharides of different molecular weight were prepared by acid hydrolysis, physiochemical properties of which were investigated through phenol sulfuric acid method, m-hydroxydiphenyl method, Lorry method and BCA method. Saccharide component was determined through HPLC, and methylation and GC-MS were used to confirm glycosidic bond pattern of neutral monosaccharides. Conduct intravenous injection of normal mice and tumor-bearing mice, and observe toxicity reducing ability of tremella polysaccharide with different molecular weights and branch types on antitumor drugs. Result: Optimum extraction technology drawn from orthogonal experiment is described below. Boil samples with 50 times of water for 3 times, and each time for 3 hrs. Tremella polysaccharides of 200,000 Dalton, 30,000 Dalton and 4,000 Dalton were prepared through acid hydrolysis. Physiochemical properties were determined. Molecule of 200,0000 Dalton contains 76.5% of neutral saccharides, 19.8% of acid saccharides and 9% of proteins. Molecule of 30,000 Dalton contains 78.2% of neutral saccharides 22.1% of acid saccharides and 6.2% of proteins. Molecule of 4,000 Dalton contains 83.1% of neutral saccharides 22.3% of acid saccharides and 8.2% of proteins. HPLC analysis shows, the above mentioned three molecules are composed of Man, Glu, Xyl, as well as small amount of 1→4 linked Xyl. It can be concluded from structure-activity analysis and pharmacological actions of tremella polysaccharide that molecule of 30,000 Dalton promotes leukocytes better when treating antitumor drugs caused decrease of peripheral blood leukocytes. Conclusion: This experiment investigates isolation, physiochemical properties, structure-activity relationship, and pharmacology of tremella polysaccharide. Sugar fractions of tremella polysaccharide which decreases toxicity of antitumor drugs and promotes leukocyte, were screened. Structure-activity relationship and function mechanism of tremella polysaccharide were summarized, which is of importance to drug development. Key words: tremella polysaccharide, physiochemical properties, PMP derivation, HPLC analysis, GC-MS |
2楼2011-04-28 22:01:43