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xukun1176Òø³æ (СÓÐÃûÆø)
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½«ËáÐÔµ°°×øø½â²úÎï¾¹ýSP-Sephadex C-25Ç¿ÑôÀë×Ó½»»»É«Æ×¡¢Sephadex G-50Äý½º¹ýÂËÉ«Æ×ºÍ·´Ïà¸ßЧҺÏàÉ«Æ×µÈ²½Ö裬´ÓöèÓãÆ¤Ã÷½ºÖзÖÀëµ½Ò»ÖÖ·Ö×ÓÁ¿Îª1356.3DaµÄ¿¹Ñõ»¯ëÄKe-¢¢£¬µ±ÆäŨ¶ÈΪ125¦Ìg/mlʱ£¬¶ÔDPPH×ÔÓÉ»ùµÄÇå³ýÂÊÄܹ»´ïµ½62.20%¡£ ΪÁ˽øÒ»²½·ÖÀë¸ü¶àµÄ½ºÔ¶àëÄ£¬ÓÖ²ÉÓó¬Â˼¼Êõ¶ÔËáÐÔµ°°×øø½â²úÎïÖзÖ×ÓÁ¿Ð¡ÓÚ3500DaµÄµÄ×é·Ö½øÐгõ·ÖÀ벢ŨËõ£¬¶³¸Éºó¼ÌÐø²ÉÓúÍ֮ǰÏàͬµÄÉ«Æ×ÊֶνøÐзÖÀ룬×îºóµÃµ½Èý¸ö¿¹Ñõ»¯»îÐÔ×îÇ¿µÄ½ºÔ¶àëÄB-¢óa ¡¢B-¢ócºÍB-¢ód¡£¾C18·´Ïà¸ßЧҺÏàÉ«Æ×¼ø¶¨£¬ÕâÈý¸ö¶àëÄ×é·Ö¶¼»ù±¾±íÏÖΪµ¥Ò»·å¡£½Ó×Å£¬ÔÙͨ¹ýESI Q-TOF MS¶ÔËüÃǽøÐмø¶¨£¬½á¹û±íÃ÷B-¢óa ¡¢B-¢óc¡¢B-¢ód·Ö±ðΪ·Ö×ÓÁ¿Îª944.5¡¢784.5ÒÔ¼°812.5µÄ¶àëÄ¡£ÆäÖУ¬B-¢ód¾°±»ùËáN¶Ë²âÐò£¬µÃÖªÆäΪº¬10¸ö°±»ùËᣬÐòÁÐΪGly-Thr-X-Gly-Ala-Y-Gly-Pro-Z-Gly£¨Î´ÍêÈ«¹«¿ª£©µÄ¶àëÄ¡£ |
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ljason
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- ·ÒëEPI: 30
- Ó¦Öú: 404 (˶ʿ)
- ½ð±Ò: 381.5
- É¢½ð: 24720
- ºì»¨: 229
- ɳ·¢: 595
- Ìû×Ó: 78709
- ÔÚÏß: 2205Сʱ
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- ×¢²á: 2009-11-16
- רҵ: ·ÖÀë¹ý³Ì
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| In order to further separation more collagen peptides, acid hydrolysis products of molecular weight less than 3500Da was separated and concentrated by ultrafiltration technology. the three strongest antioxidant activity of the collagen peptide B-¢ó a, B-¢ó c and B-¢ó d was obtained via lyophilize and chromatographic separation. the three polypeptide components were performed a single peak when identification by RP-HPLC C18. the molecular weight of the peptide B-¢ó a, B-¢ó c and B-¢ó d were 944.5,784.5 and 812.5 obtained from ESI Q-TOF MS identification. The B-¢ó d was contained 10 amino acids detected by amino acid N terminal sequencing and the sequence was Gly-Thr-X-Gly-Ala-Y-Gly-Pro-Z-Gly (not fully open). |
3Â¥2011-04-13 15:03:42
ljason
гæ (ÎÄѧ̩¶·)
Íâ¸ÕÄÚÈáÖ®åÐÒ£´ï×Ó
- ·ÒëEPI: 30
- Ó¦Öú: 404 (˶ʿ)
- ½ð±Ò: 381.5
- É¢½ð: 24720
- ºì»¨: 229
- ɳ·¢: 595
- Ìû×Ó: 78709
- ÔÚÏß: 2205Сʱ
- ³æºÅ: 904684
- ×¢²á: 2009-11-16
- רҵ: ·ÖÀë¹ý³Ì
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xukun1176(½ð±Ò+100, ·ÒëEPI+1): 2011-04-19 10:22:28
| µÚÒ»¶Î£ºAntioxidant Peptide Ke-¢¢ with a molecular weight of 1356.3Da was isolated from shark skin gelatin after the characterization SP-Sephadex C-25 strong cation exchange chromatography, Sephadex G-50 gel filtration chromatography and RP-HPLC of acid protease enzyme product. The scavenging ratio could reach 62.20% when its concentration was 125¦Ìg/ml. |
2Â¥2011-04-13 14:43:36
qingkong0918
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- ×¢²á: 2010-10-16
- ÐÔ±ð: MM
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xukun1176(½ð±Ò+100): 2011-04-19 10:22:37
| The zymohydrolysised product of acid protease is processed by Strong cation exchange SP-Sephadex C-25 , gel filtration chromatography Sephadex G-50 and reversed-phase high-performance liquid chromatography£¬and anti-oxidant peptide is obtained£¬whose molecular weight is 1356.3Da¡£ Rate of clearing free radical DPPH can reach 62.20% when the concentration is 125¦Ìg/ml . In order to separate more collagenous polypeptide£¬ those whose molecular weight are less than 3500Da are separated and condensed initially by hythseperfiltration, then are ayophilized£¬seperated by the same chromatographs as above, three collagenous polypeptides with the most strong anti-oxidant activity are obtained, B-¢óa ¡¢B-¢ócºÍB-¢ód. Assessed by C18 reversed-phase high-performance liquid chromatography , the three polypeptides all present simple spike. The molecular weight of the three polypeptides are 944.5¡¢784.5 and 812.5 respectively. Especially , B-¢ód is assessed to have 10 amino acids , N terminal amino acid sequence is Gly-Thr-X-Gly-Ala-Y-Gly-Pro-Z-Gly£¨not fully publicized£©. |

4Â¥2011-04-13 15:37:38













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