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wd519wd706

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Limulus polyphemus and other horseshoe crabs defend themselves against infection and physical damage with a clot-forming, zymogen-cascade. The inactive
components of the cascade are sequestered in intracellular granules of the circulating amebocytes . When the hemolymph comes in contact with the lipopolysaccharide (LPS) of Gram-negative bacterial cell walls or（1-3）-β-D-glucans of fungal cell walls , the amebocytes degranulate, releasing the proteins of the cascade.The LPS then activates Factor C, while the （1 -3）-β-D-glucan activates Factor G of the zymogen cascade resulting in clot formation from the cleavage of coagulogen. Amebocytes also contain an LPS-binding protein known as the Limulus anti-lipopolysaccharide factor (LALF) . The amino-acid sequence of L.
polyphemus LALF was the basis for making thesynthetic gene, the product of which is called endotoxin neutralizing protein (rENP). The natural product
(LALF) and rENP are sold commercially for neutralizing endotoxin either as puriﬁed proteins or covalently attached to solid-support devices.

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1楼2011-04-06 13:49:50

已阅回复此楼关注TA 给TA发消息送TA红花 TA的回帖

propanediol

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sltmac(金币+20, 翻译EPI+1): 谢谢，欢迎常来~~~ 2011-04-17 12:30:40

引用回帖:

Originally posted by wd519wd706 at 2011-04-06 13:49:50:
Limulus polyphemus and other horseshoe crabs defend themselves against infection and physical damage with a clot-forming, zymogen-cascade. The inactive
components of the cascade are sequestered in ...

马蹄蟹和其它大螃蟹通过凝血形成，酶原级联抵抗感染和物理伤害，无活性的级联化合物被隔离在循环阿巴米状细胞内的液泡中，当血淋巴与革兰阴性细菌细胞壁脂多糖（LPS）或真菌细胞壁的（1-3）-β-的D -葡聚糖接触,阿巴米状细胞排出液泡，释放其中级联蛋白，壁脂多糖激活因子C，而（1 -3）-β- D -葡聚糖激活酶原级联因子G,切断原酶原形成凝血。阿巴米状细胞还具有鲎抗内毒素因子（LALF），LALF的氨基酸序列是合成基因的基础，其产品被称为内毒素中和蛋白（rENP）。商业的天然产物（LALF）和rENP以纯化蛋白或共价链接到载体的方式被用来中和内毒素。

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