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B. cinerea was isolated from decayed tomato fruit and incubated on potato dextrose agar medium (PDA). Spore suspensions of the strain were prepared by flooding the 7-d-old culture dishes incubated at 25±1 ◦C with 0.05% Tween-80 solution. The spore suspension was adjusted to 1×106 conidia/mL with a hemocytometer. The inoculations were carried out in a sterilized laminar flow cabinet 1, 3, 6, 9 and 12 d after fruit were treated. Ten fruit per replicate and three replicates per treatment were surface-sterilized by immersion in 70% ethanol for 1 min, rinsed three times in sterile distilled water, and then wounded with a sterile nail at three points (4mm deep×2mm wide) on the equator of each fruit. Then 10uL of spore suspension was injected into each wound site and the fruit incubated at 25±1 ◦C, 85–90% RH. Disease symptoms on each fruit were recorded on the 3rd day after inoculation (when the symptoms were visible and countable).Lesion size = 3.14×(lesion diameter/2)2. |
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昔年残梦(金币+15, 翻译EPI+1): 2011-04-01 18:35:22
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B. cinerea was isolated from decayed tomato fruit and incubated on potato dextrose agar medium (PDA). Spore suspensions of the strain were prepared by flooding the 7-d-old culture dishes incubated at 25±1 ◦C with 0.05% Tween-80 solution. 从腐烂的番茄果实内分离出灰霉病菌并在PDA（马铃薯葡萄糖琼脂培养基）上进行培养。将该菌株的孢子悬浮液充满已制备7 天的培养皿中，用0.05%非离子活性剂-80溶液在25度左右进行温育。 |
3楼2011-04-01 18:32:17
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