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金虫 (小有名气)

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虫号: 456712

[交流] 【求助/交流】求问从西格玛买的连霉亲和素修饰的金纳米粒子怎么用

我是学化学的，刚从sigma买了链霉亲和素capped金纳米粒子，，说明书上说稀释了才能用，但是稀释的具体操作我看不懂，外行真可悲。。。

哪位虫友知道请指点一下
This product should be diluted for most applications.
It is recommended that the diluent buffer contain
0.15 M saline buffered at pH 6 to 8, with 0.5% albumin
(Product No. A 7638) and 0.05% TWEEN 20 to
minimize background (additional buffer supplement
may be required for certain applications e.g., see "dot
blot" diluent). It is also recommended that prior to
application, the diluted conjugate be allowed to
equilibrate at least 20 minutes in lower glycerol content.
Optimum concentration of the conjugate must be
determined empirically dependent on specific usage
and generally may range from final A 520 = 1.0 to 0.05
(1:2.5-1:50 dilution) with incubation times ranging from
30 minutes to 12 hours.

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