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youcai147

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[交流] 【求助/交流】问一个跑胶的问题

跑胶的时候，想要图上的第二条带的产物，跑胶的方向如图，文献上的有两个东西要插入胶中，我就是想理解下这个 glassfiber microfilters 和dialysis membranes 是不是我图上那样的前面顺序，还是先放d再放g

文献的原文是：Nanocrystal molecule purification: Agarose gels (3 ± 4%) were used to analyze and isolate the desired structures. Tris-borate EDTA (1X, TBE)
was used as the running buffer. Glycerol loading buffer (6X) was used to load the samples onto the gels.[18] After electrophoresis, samples were isolated by slicing the gel in front of the desired band and inserting glassfiber microfilters (Whatman GF/C) that were backed with dialysis membranes (Spectra/Por, MWCO: 10000). The bands were incorporated into the paper by electrophoresis. The filter/membrane pieces were then placed with the filter facing down into 0.5-mL Eppendorf tubes which had been pierced at the bottom with 26-gauge needles. These Eppendorf tubes were fitted into 1.5-mL Eppendorf tubes and centrifuged for one minute (14 000_g) to isolate the desired products. Syringe tip filters (Millipore, 0.45 mm) were used to remove residual glass fibers from the resulting solutions. Typically, isolated yields were 30 ± 40% (dimers) and 20 ± 30% (trimers) based on the nanocrystal starting materials.

[ Last edited by youcai147 on 2011-3-24 at 09:32 ]

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1楼 2011-03-23 17:23:55

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天使托

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youcai147(金币+2): 谢谢了，就是想知道文献中back这个词的理解 2011-03-23 17:49:51

说实话，不懂，帮顶！

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2楼2011-03-23 17:41:47

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321wangke321

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Originally posted by youcai147 at 2011-03-23 17:23:55:

跑胶的时候，想要图上的第二条带的产物，跑胶的方向如图，文献上的有两个东西要插入胶中，我就是想理解下这个 glassfiber microfilters 和dialysis membranes 是 ...

听不懂啊

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3楼2011-03-23 18:34:11

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silicare

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★ ★
lstt09nk(金币+2): 感谢参与 2011-03-23 19:29:41
youcai147(金币+5): 非常感谢，我再好好看看 2011-03-24 09:31:07
youcai147(金币+2): 我在原贴上贴了上下文，感觉还是插了两个东西 2011-03-25 08:55:46

不是插两个，只插玻璃纤维滤膜

建议你还是把前后文，上下文好好读读。
感觉应该是在胶的前方插入玻璃纤维滤膜，继续跑胶，让滤膜拦截DNA，然后取出滤膜，用透析的方法回收DNA，这应该是整片文章的主旨所在