| 查看: 1110 | 回复: 6 | |||
396101466金虫 (正式写手)
一段遗失的美好
|
[交流]
【求助/交流】有关免疫沉淀电泳的的操作 已有5人参与
|
| 最近看了一篇文章，做了免疫沉淀分析，并作了电泳，但是不清楚电泳中的的成分是沉淀还是上清，想问各位高人，免疫沉淀电泳的具体操作是怎么样的！ |
回复此楼» 猜你喜欢
22408 调剂材料
已经有6人回复
-
285求调剂
已经有12人回复
-
求调剂
已经有6人回复
-
085600材料与化工301分求调剂院校
已经有19人回复
-
277工科求调剂
已经有11人回复
-
277求调剂 数一104分
已经有13人回复
-
304求调剂
已经有10人回复
-
336求调剂，一志愿中科大
已经有6人回复
-
071000生物学，一志愿深圳大学296分，求调剂
已经有3人回复
-
一志愿北京化工085600 310分求调剂
已经有19人回复
» 本主题相关价值贴推荐，对您同样有帮助:
- 【求助/交流】求助~~~关于免疫共沉淀~~~
已经有8人回复
- 10BB求生化分离技术电子版 田亚平 等编 化学工业出版社
已经有5人回复
yabxxh
木虫 (正式写手)
- BioEPI: 11
- 应助: 22 (小学生)
- 贵宾: 0.005
- 金币: 2238
- 散金: 98
- 红花: 9
- 帖子: 465
- 在线: 88.6小时
- 虫号: 157001
- 注册: 2006-01-05
- 专业: 生物物理、生物化学与分子
2楼2011-03-23 11:05:22
396101466
金虫 (正式写手)
一段遗失的美好
- 应助: 26 (小学生)
- 金币: 2211.2
- 散金: 230
- 红花: 3
- 帖子: 515
- 在线: 150.3小时
- 虫号: 1220821
- 注册: 2011-03-04
- 性别: GG
- 专业: 食品科学基础
★ ★ ★
silicare(金币+3): 很好，鼓励交流 2011-04-25 18:23:23
silicare(金币+3): 很好，鼓励交流 2011-04-25 18:23:23
|
我只想解释两个图，图的说明是Fig. 5. Immunoprecipitation using the D1 (A) and A3 (B) Fab fragments and cow's milk samples. The Fab fragments were immobilised on protein L beads and then incubated with themilk samples without orwith heat treatment (0,15, 30 or 60 min at 95°C). After washing steps, the samples were analysed on a 15% SDS-PAGE gel and stained with silver. The boxes indicate the protein bands that were cut out for MS-analysis. Arrows indicate the Fab fragments. Ctr=protein L beads without the immobilized Fab incubated with 0′ (1) and 60′ (2) heated BLG sample. As a control, 1 µg of native BLG (Sigma) and D1/A3 Fab were used. 其操作内容是： Pasteurized, skimmed cow's milk was centrifuged (20 000×g, 2 min, 4 °C) and the supernatant was diluted 1:4 in PBS and used for immunoprecipitation. In the immunoprecipitation assay, both heat-treated (15, 30,60 min at 95 °C) and native cow's milk samples were used. The BLG-specific Fab fragments D1 and A3 were immobilized onto protein L beads (CBind™L, Fluka) according to the manufacturer's instructions (100-150 µg protein/25 µl beads). Then the milk samples were incubated with protein L-Fab beads for 1 h at room temperature. After the washing step with PBS-0.05% Tween 20, elution was performed by adding first 25 µl and then 50 µl of 0.1 M glycine-HCl (pH 2). The eluted samples were separated on a 15% SDS-PAGE gel and stained with silver. 我想问的是图中的条带怎么解释呀？谢谢啦！   |
3楼2011-03-23 15:22:59
350784478
木虫 (正式写手)
- 应助: 3 (幼儿园)
- 贵宾: 0.882
- 金币: 1255.8
- 散金: 12
- 红花: 3
- 帖子: 835
- 在线: 91.4小时
- 虫号: 576561
- 注册: 2008-06-20
- 性别: GG
- 专业: 遗传学研究新技术与方法
4楼2011-04-25 17:52:36
ujsyangyong
铁杆木虫 (著名写手)
- BioEPI: 1
- 应助: 157 (高中生)
- 金币: 12087.9
- 红花: 6
- 帖子: 1002
- 在线: 134.7小时
- 虫号: 471632
- 注册: 2007-12-02
- 性别: GG
- 专业: 脑、脊髓、周围神经损伤及
5楼2011-04-25 22:10:28
396101466
金虫 (正式写手)
一段遗失的美好
- 应助: 26 (小学生)
- 金币: 2211.2
- 散金: 230
- 红花: 3
- 帖子: 515
- 在线: 150.3小时
- 虫号: 1220821
- 注册: 2011-03-04
- 性别: GG
- 专业: 食品科学基础
6楼2011-04-27 19:26:21
7楼2013-01-26 21:31:02
