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B. cinerea was isolated from decayed tomato fruit and incubated on potato dextrose agar medium (PDA). Spore suspensions of the strain were prepared by flooding the 7-d-old culture dishes incubated at 25±1 ◦C with 0.05% Tween-80 solution. The spore suspension was adjusted to 1×106 conidia/mL with a hemocytometer. The inoculations were carried out in a sterilized laminar flow cabinet 1, 3, 6, 9 and 12 d after fruit were treated. Ten fruit per replicate and three replicates per treatment were surface-sterilized by immersion in 70% ethanol for 1 min, rinsed three times in sterile distilled water, and then wounded with a sterile nail at three points (4mm deep×2mm wide) on the equator of each fruit. Then 10uL of spore suspension was injected into each wound site and the fruit incubated at 25±1 ◦C, 85–90% RH. Disease symptoms on each fruit were recorded on the 3rd day after inoculation (when the symptoms were visible and countable).Lesion size = 3.14×(lesion diameter/2)2. [ Last edited by 昔年残梦 on 2011-3-21 at 22:15 ] |
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昔年残梦(金币+10, 翻译EPI+1): 2011-03-22 22:24:20
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灰霉病菌分离出番茄果实腐烂和培养马铃薯葡萄糖琼脂培养基（PDA）的。该菌株孢子悬浮液，制备了洪水7 d的培养，在25 ± 1℃温育◦为0.05％吐温80溶液的菜肴。孢子悬液调整为1 × 106个/毫升与血球。在接种进行了消毒柜层流出来1，3，6，9和12 d后果实治疗。十大水果，每重复每个处理三个重复进行表面消毒70％乙醇浸泡1分钟，在无菌蒸馏水冲洗三次，然后在三个点受伤（4毫米× 2毫米深宽）用无菌钉在赤道每个果实。然后孢子悬浮液注射到每个10uL伤口在25 ± 1◦ç，相对湿度85-90％孵育水果。 对每个水果病症状记录在接种后第3天（当症状明显，可数）。病灶大小= 3.14 ×（病灶直径/ 2）2。 |
2楼2011-03-22 14:39:58
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昔年残梦3楼
2011-04-01 18:11
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