| 查看: 565 | 回复: 5 | |||
| 本帖产生 1 个 翻译EPI ，点击这里进行查看 | |||
[交流]
翻译
|
|||
|
The ACC oxidase (ACO) activity was measured as the ability of plant tissue to convert exogenous ACC to ethylene. The homogenate was prepared in an extraction buffer as described byMoya-Leon and John (1994), with modifications. One g tomato pericarp tissue was homogenized using a cold pestle and mortar with 3mL extraction buffer which contained 0.1MTris–HCl (pH7.5), 5%PVP, 10%glycerol, 5.0 mM dithiothreitol, 0.1 mM FeSO4 and 30 mM sodium ascorbate. The homogenate was centrifuged at 10,000×g for 20min at 4 ◦C.The determination of ACO activity was carried out in sealed tubes with a reaction mixture containing 1.8mL of 0.1M Tris–HCl buffer (pH 7.5, containing 10% glycerol, 30mM sodium ascorbate, 30mM NaHCO3, 0.1mM FeSO4 and 1.0mM ACC) and 0.2mL of enzyme extract. After being incubated at 30 ◦C for 20 min, 1mL of the gaseous portion was removed and assayed for ethylene as described above. The ACO activity was expressed as nmol g−1 FWh−1. Extracts for lipoxygenase (LOX) activity assays were prepared according to the method of Surrey (1964), with slight modifications. Two g tomato pericarp tissue was homogenized using a cold pestle and mortar in 10mL of 50mM phosphate buffer (pH 7.0) at 4 ◦C and centrifuged at 10,000×g for 15min. The supernatant liquid was used as the enzyme source. The reaction mixture contained 25 L of 100mM linoleic acid sodium, 2.775mL of 100mM acetic acid buffer (pH 5.5) and 0.2mL of supernatant enzyme extract at 30 ◦C. A unit of LOX was defined as a change of 1 absorbance unit at 234 nm in 1 min using a UVIKON XL Ultraviolet spectrophotometer (Secomam, France). These determinations were made from three replicates sampled from 5 fruit. |
回复此楼» 猜你喜欢
298求调剂
已经有4人回复
-
生物学308分求调剂（一志愿华东师大）做过分子实验
已经有7人回复
-
288求调剂，一志愿华南理工大学071005
已经有11人回复
-
一志愿深大085601材料工程专业（专硕）300分可以调剂去哪
已经有13人回复
-
一志愿郑州大学085600求调剂
已经有24人回复
-
327求调剂
已经有12人回复
-
327求调剂
已经有10人回复
-
材料考研求调剂总分280
已经有28人回复
-
308求调剂
已经有14人回复
-
材料专硕调剂
已经有13人回复
» 抢金币啦！回帖就可以得到:
-
长江大学化学与环境工程学院2026年硕士研究生调剂公告（理学、工学均可）
+5/785
-
哈尔滨工程大学（青岛）动力工程及工程热物理专业招聘博士1名
+1/486
-
2007年一起玩耍的老虫子们，想死你们啦~~~出来冒个泡吧……
+2/188
-
物理学 调剂
+1/91
-
【考研调剂】华侨大学发光材料与信息显示研究院接收专硕和学硕调剂
+1/41
-
浙师大化与材学院2026年有大量硕士研究生调剂名额 （线上 调剂）
+1/41
-
河北大学无机化学专业招收调剂生
+1/37
-
南京地区，有要戴森洗地机吗？仅限自取
+1/35
-
【硕士调剂】【分子医学 10医类 最后机会】2026年青岛大学硕士调剂补招
+1/21
-
南京，真诚征女友
+1/11
-
齐鲁工业大学-材料方向招收两名专硕调剂
+1/11
-
福建师范大学 2026硕士调剂 （化学、光电器件、物理、纳米材料方向）
+1/8
-
2026年西华大学材料学院-材料近净成形与表面技术研究团队-招收调剂研究生
+1/5
-
杭州师范大学-浙江省湿地智慧监测与生态修复重点实验室团队硕士调剂公告
+1/4
-
【#上海调剂急录#】能接受985联合培养的速来！带你发一区文章！
+1/4
-
【上海调剂】985联合培养！别错过能送你去丹麦/交大/同济/中科院的神仙导师！
+1/4
-
海水鱼类遗传育种课题组招收硕士调剂生
+1/4
-
齐齐哈尔大学李莉课题组诚招2026级考研调剂生（学硕和专硕）
+1/3
-
合工大具身智能柔性机器人方向招博士
+1/3
-
线上复试：河南理工大学 化工学院 接收调剂硕士研究生
+1/2
昔年残梦(金币+2, 翻译EPI+1): 2011-03-20 13:50:38
|
The ACC oxidase (ACO) activity was measured as the ability of plant tissue to convert exogenous ACC to ethylene. The homogenate was prepared in an extraction buffer as described by Moya-Leon and John (1994), with modifications. ACC氧化酶（ACO）的活性测定可以用来衡量植物组织将外源的ACC转化为乙烯的能力。这种匀浆由Moya-Leon和约翰在1994年提取的一种缓冲液中制备、描述并加以修饰。 |
2楼2011-03-20 13:39:44
昔年残梦(金币+4): 2011-03-20 13:52:09
|
One g tomato pericarp tissue was homogenized using a cold pestle and mortar with 3mL extraction buffer which contained 0.1MTris–HCl (pH7.5), 5%PVP, 10%glycerol, 5.0 mM dithiothreitol, 0.1 mM FeSO4 and 30 mM sodium ascorbate. The homogenate was centrifuged at 10,000×g for 20min at 4 ◦C. The determination of ACO activity was carried out in sealed tubes with a reaction mixture containing 1.8mL of 0.1M Tris–HCl buffer (pH 7.5, containing 10% glycerol, 30mM sodium ascorbate, 30mM NaHCO3, 0.1mM FeSO4 and 1.0mM ACC) and 0.2mL of enzyme extract. 1克番茄果皮组织匀浆的制备需要用冷的研钵和杵拌着3毫升的提取缓冲液进行研磨，缓冲液中包含有0.1摩Tris -盐酸（pH7.5），5％的PVP，10％甘油，5.0毫摩二硫苏糖醇，0.1毫摩硫酸亚铁和30毫摩抗坏血酸钠。将这个匀浆在4度下，10，000转离心20分钟克。 ACO活性的测定：将其放在一个装有混合物的密封管中，混合物中包含有1.8毫升的0.1摩的Tris - HCl缓冲液（pH 7.5 ，含10％甘油，30毫摩抗坏血酸钠，30毫摩碳酸氢钠，0.1毫摩硫酸亚铁和1.0毫摩ACC）和0.2毫升酶提取物。 |
3楼2011-03-20 13:50:44
昔年残梦(金币+10): 2011-03-20 14:03:54
|
After being incubated at 30 ◦C for 20 min, 1mL of the gaseous portion was removed and assayed for ethylene as described above. The ACO activity was expressed as nmol g−1 FWh−1.Extracts for lipoxygenase (LOX) activity assays were prepared according to the method of Surrey (1964), with slight modifications. Two g tomato pericarp tissue was homogenized using a cold pestle and mortar in 10mL of 50mM phosphate buffer (pH 7.0) at 4 ◦C and centrifuged at 10,000×g for 15min. 30度温育20分钟，其中1毫升的气态部分被去除，就能够检测到如上所述的乙烯成分。ACO的活性表现为nmol g−1 FWh−1。根据萨里法（1964年）所提出的方法，脂氧合酶（LOX）活性检测的提取物被制备，但略有修改。两g番茄果皮组织匀浆使用上述同样方法，用10毫升50毫摩磷酸盐缓冲液（pH值7.0）在4◦ç，10000 ×转下离心15分钟。 |
4楼2011-03-20 13:58:15
5楼2011-03-20 14:01:45
昔年残梦(金币+14): 2011-03-20 14:11:16
|
The supernatant liquid was used as the enzyme source. The reaction mixture contained 25 L of 100mM linoleic acid sodium, 2.775mL of 100mM acetic acid buffer (pH 5.5) and 0.2mL of supernatant enzyme extract at 30 ◦C. A unit of LOX was defined as a change of 1 absorbance unit at 234 nm in 1 min using a UVIKON XL Ultraviolet spectrophotometer (Secomam, France). These determinations were made from three replicates sampled from 5 fruit. 这个上清液可作为酶源。这个反应混合物中含有25升100毫摩亚油酸钠，2.775mL 100毫摩醋酸缓冲液（pH 5.5）和0.2毫升，30度的酶提取液。单位LOX被定义为：一分钟使用双光束紫外可见分光光度计在234纳米出的吸光值（Secomam，法国）。 这些测定结果是从5种水果中进行3次重复采样所的到的。 |
6楼2011-03-20 14:05:40
