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| ACC content was measured by the method of Lizada and Yang (1979). Three g tomato pericarp tissue was homogenized using a cold pestle and mortar with 0.5 g polyvinylpyrrolidone in 10mL of 80% ethanol at 4◦C and centrifuged at 10,000×g for 25min. The supernatant was evaporated under vacuum at 50◦C. Residues were dissolved in 5mL of distilled water and 2.5mL of chloroform. After being stored at 4◦C overnight, 1mL of aqueous solution was mixed with 0.1mL HgCl2 (80mM) and 2.5mL distilled water in test tubes. The tubes were sealed with a stopper and rubber septum and 0.4mL 5% NaOH–NaClO solution (1:2, v/v) was injected into the tubes, shaken and incubated for 2 min. One mL of the gaseous portion was removed and assayed for ethylene as described above. ACC content was expressed as n mol g−1 FW h−1. This determination was made from three replications using five different fruit per replication. |
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昔年残梦(金币+6, 翻译EPI+1): 2011-03-20 12:45:11
| ACC（1-氨基环丙烷羧酸）含量用Lizada和yang（1979）等的方法测定。在4◦C 下，3g西红柿果皮组织在10ml含有0.5g聚乙烯吡咯烷酮的80%乙醇中用冷研杵以及研钵磨匀，并在10,000×g下离心25分钟。 上层液体在50◦C下真空挥发干燥，残留物然后溶于5ml蒸馏水和2.5ml氯仿中。4◦C 下放置过夜后，在试管中将1ml该水溶液与0.1ml HgCl2 (80mM) 和2.5ml蒸馏水混合。 所有试管均用塞子和橡胶膜封闭，然后将0.4mL 体积比为1:2的5% NaOH–NaClO溶液注射入这些试管中，振摇，培养2分钟。移取1ml气相部分并按上面描述方法测定乙烯含量。ACC含量用n mol g−1 FW h−1表达。这种测定每次用5种不同的水果，每个测定需进行三次重复测定。 |
2楼2011-03-20 00:15:15
