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2.2.2. Microbial perchlorate reduction kinetics Three BOD bottles containing the same amount of biomass and 300 ml of culture medium each were spiked with 24.1 mg/L of perchlorate and 500 mg/L of acetate. The bottles were sealed and shaken by the same methods as above. Triplicate samples (500ul for each) were taken from the 3 BOD bottles at different times. The bottles were then filled up with DI (deionized) water, sealed again, and shaken again in the incubator. All samples were analyzed to measure the residual perchlorate concentrations. 主要不理解倒数第二句的意思,是再加入500微升的样品,把培养液重新补充到300毫升吗? |
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