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wwdxx2004

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[交流] 【求助/交流】求助intracellular volume什么意思

请问intracellular volume什么意思?怎么测定???

[ Last edited by 1949stone on 2011-3-14 at 17:01 ]
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zhengfan1109

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wwdxx2004(金币+1): 谢谢!请问具体要怎样测定呐?? 2011-03-14 19:11:17
引用回帖:
Originally posted by wwdxx2004 at 2011-03-14 16:17:49:
请问intracellular volume什么意思?怎么测定???

[ Last edited by 1949stone on 2011-3-14 at 17:01 ]

细胞内体积?
即细胞的体积?
测定细胞半径,将细胞当成球形模型,利用球体公司算细胞体积?
2楼2011-03-14 18:39:44
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shawnfang

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amisking(金币+1): 鼓励发帖交流。 2011-03-14 20:10:43
wwdxx2004(金币+2): 谢谢 2011-03-14 22:41:07
胞内体积。
这是一个专利的摘要,希望对你有帮助:
A method and apparatus for measuring changes in cell volume generally includes introducing cells into a chamber having a volume between 2 and 100 times the volume of the introduced cell. A first electrically conductive extracellular fluid is introduced into the chamber and a current is applied. The current flow is measured. The first fluid is exchanged with a second electrically conductive extracellular fluid and a current is applied. The current flow is measured. The first current flow result and the second current flow result are used in conjunction with known current flows to monitor changes in the volume corresponding to fluid flow between the cell and an extracellular fluid.
3楼2011-03-14 19:12:53
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zhengfan1109

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wwdxx2004(金币+2): 谢谢! 2011-03-14 22:41:18
引用回帖:
Originally posted by zhengfan1109 at 2011-03-14 18:39:44:
细胞内体积?
即细胞的体积?
测定细胞半径,将细胞当成球形模型,利用球体公司算细胞体积?

你要先确认你的细胞模型可以用球形模拟与否,如果可以,在显微镜下观察细胞,同时有软件可以直接测定细胞半径的(你可以查下),估算出半径,然后利用球形体积公式算出体积。
但是这个方法的误差可能比较大。
4楼2011-03-14 20:02:17
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