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小木虫论坛-学术科研互动平台» 学术交流区» 论文翻译 » 论文翻译求助完结子版 » 摘要翻译
北京石油化工学院2026年研究生招生接收调剂公告
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shawne2008

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Downstream Antibody Purification using Aqueous Two-Phase Extraction

The extraction of antibodies using a polyethyleneglycol(PEG)-citrate aqueous two-phase system(ATPS) was in vestigated.Studies using purified monoclonal antibody(mAb) identified operating ranges for successful phase formation and factors that significantly affected antibody partitioning. The separation of antibody and host cel lprotein(HCP) from clarified cell culture media was examined using statistical design of experiments(DOE). The partitioning of antibody was nearly complete over the entire range of the operating space examined.A model of the HCP partitioning was generated in which both NaCl and citrate concentrations were identified assignificant factors.To achieve the highest purity,the partitioning of HCP
from cell culture fluid into the product containing phase was minimized using a Steepest Descent algorithm.An optimal ATPS consisting of 14.0%(w/w) PEG,8.4%(w/w)citrate,and7.2%(w/w)NaCl at pH7.2 resulted in a product yield of 89%,anapproximate 7.6- fold reduction in HCP levels relative to the clarified cell culture fluid before extraction and an overall purity of 70%.A system consisting of 15%(w/w)PEG,8%(w/w)citrate,and15% (w/w) NaCl at pH5.5 reduced product-related impurities(aggregates and low molecular product fragments)from 40% to less than0.5%while achieving 95% product recovery.At the experimental conditions that were optimized in the batch mode,a scale-up model for the use of counter-current extraction technology was developed to identify potential improvements in purity and recovery tha tcould be realized in the continuous operational mode
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1楼 2011-03-13 15:34:53
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爱与雨下

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The separation of antibody and host cel lprotein(HCP) from clarified cell culture media was examined using statistical design of experiments(DOE).使用实验（DOE）的统计设计对从分离细胞培养基上得到的抗体和宿主细胞蛋白质(HCP)进行检测。
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5楼2011-03-13 16:06:26
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爱与雨下

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shawne2008(金币+120, 翻译EPI+1): 一起评了吧，整体上还是比较满意的，但是部分还是不够准确，感谢应助 2011-03-13 21:51:57
Downstream Antibody Purification using Aqueous Two-Phase Extraction
利用水溶液两相萃取的方法对下游抗体的纯化
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2楼2011-03-13 15:53:19
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爱与雨下

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The extraction of antibodies using a polyethyleneglycol(PEG)-citrate aqueous two-phase system() was in vestigated.

研究了用聚乙二醇（PEG）-柠檬酸双水相体系（ATPS）提取抗体
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3楼2011-03-13 15:55:06
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爱与雨下

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Studies using purified monoclonal antibody(mAb) identified operating ranges for successful phase formation and factors that significantly affected antibody partitioning. 研究使用纯化单克隆抗体（mAb）识别操作包括相的成功形成和影响抗体分离的因素
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4楼2011-03-13 15:59:40
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