Originally posted by 0610616 at 2011-03-09 19:22:07:
GG很厉害呀

Originally posted by wangleisdnu at 2011-03-09 19:28:18:
is this suggestion helpful for you

Originally posted by sxxuan at 2011-03-09 16:23:36:
回收之后我一般都不跑胶了，因为试过几次，纯化后跑胶没条带，但是连接转化又能得到阳性克隆
真是不可思议的实验

Originally posted by wangleisdnu at 2011-03-09 15:40:38:
1 you did not cut the right band
2 the column for purification is not efficient for the plasmid purifiy
3 you used less amount of elution solution to elute your plasmid