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Green vegetables such as broccoli florets and asparagus spears are young, actively
growing tissues and consequently have high metabolic and respiratory rates when
harvested. These crops are prone to harvest-induced senescence, which is initiated
within a few hours after removal from the parent plant (King and O¡¯Donoghue, 1995;
Downs and Somerfield, 1997). The senescence program is promoted by ethylene or
dehydration and inhibited by cytokinin or sucrose, and is characterized by rapid
depletion of sugars and organic acids, proteolysis, lipid peroxidation, increases in
free amino acids and ammonia, and alteration of gene expression (King and Morris,
1994; Zhuang et al., 1995; Coupe et al., 2003; McKenzie et al., 2004; Gapper et al.,
2005). The changes in gene expression include differentially regulated induction of
at least four cysteine proteases (Coupe et al., 2003) and a marked up-regulation of
asparagine synthase as a consequence of sucrose depletion (Downs and Somerfield,
1997; Winichayakul et al., 2004). Even in the early stages, these dramatic changes
result in loss of fresh flavor and coincident increase in undesirable flavor and aroma.
The flavor acceptability of asparagus spears decreased significantly after two days
at 1 ¡ãC, with an additional decline during the subsequent five days of storage (King
et al., 1988). This loss of sensory quality was associated with off flavor development,
decreased soluble carbohydrates, and an increase in soluble protein.

Benefits of low temperature, high humidity, and controlled atmosphere (CA) or
modified atmosphere packaging (MAP) storage
The best measures to retard harvest-induced senescence and maintain fresh-like
sensory quality are rapid cooling with little delay after harvest and subsequent
maintenance of low temperature and high humidity during storage (Toivonen and
Forney, 2004; Jones et al., 2006). However, there also has been considerable
success in devising suitable CA or MAP conditions for preservation of quality in
broccoli florets up to twice as long as in air storage (Jacobsson et al., 2004;
Toivonen and Forney, 2004; DeEll et al., 2006). Jacobsson et al. (2004) found that
use of low-density polyethylene film, inclusion of an ethylene-absorbing sachet,
and a package atmosphere of 5% O , 7% CO  for MAP storage gave the best fresh-
2 2
like sensory quality of cooked broccoli. DeEll et al. (2006) showed that use of
packets including sorbitol plus potassium permanganate markedly reduced accu-
mulation of off odor fermentative volatiles in MAP broccoli during 29 days of
storage at 0¨C1 ¡ãC. These good results notwithstanding, there is always the potential
for production of malodorous volatile sulfur compounds, most notably methanethiol
and dimethyl disulphide, when O  falls below 1% or CO  exceeds 10% (Forney et
2 2
al., 1991; Hansen et al., 1992; Di Pentima et al., 1995). Quite recently, as a first
step toward elucidation of the molecular mechanisms whereby CA delays the rapid
onset of harvest-induced senescence in broccoli florets, Eason et al. (2007) used
differential display analysis to identify a set of stress response genes induced
during the first 24 hours under 10% CO , 5% O  CA.

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Benefits of low temperature, high humidity, and controlled atmosphere (CA) or
modified atmosphere packaging (MAP) storage
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