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tihommey(金币+15, 翻译EPI+1): 3Q 2011-02-28 16:45:05
Using pour plate method,suck 0.1ml culture from each tube without growth of the bacteria , drawing into sterile plates, then add 20m l 40 ℃ ~ 45 ℃ molten nutrient agar medium, mix until coagulation. Each concentration was done for triplets at 37 ℃,24h, the minimum concentration of the plate which the number of colonies on it is less than five groups is corresponding to X MBC.
Take a certain amount of X, with a double dilution method Allocation of concentration gradient. Each series were inoculated with Staphylococcus aureus, Escherichia coli, Escherichia coli pig M1, swine E. coli M2, swine Salmonella R1, Aeromonas hydrophila, Edward coli, each concentration was repeated three times. With sterile water as control, incubation at 37 ℃ for 24h, cell growth phenomenon was observed, concentration of sample in the plate with no colony growth is MIC. |
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