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【分享】在小鼠大脑中特异表达的长非编码RNA
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SPECIFIC EXPRESSION OF LONG NONCODINGRNAS IN THE MOUSE BRAIN 在小鼠大脑中特异表达的长非编码RNA PNAS January 15, 2008 摘要：A major proportion of the mammalian transcriptomecomprises long RNAs that have little or no protein-coding capacity (ncRNAs). Only a handful of such transcripts have been examined in detail, and it is unknown whether this class of transcript is generally functional or merely artifact. Using in situhybridization data from the Allen Brain Atlas, we identified 849 ncRNAs(of 1,328 examined) that are expressed in the adult mouse brain and found that the majority were associated with specific neuroanatomicalregions, cell types, or subcellularcompartments. Examination of their genomic context revealed that the ncRNAswere expressed from diverse places including intergenic, intronic, and imprinted loci and that many overlap with, or are transcribed antisense to, protein-coding genes of neurological importance. Comparisons between the expression profiles of ncRNAsand their associated protein-coding genes revealed complex relationships that, in combination with the specific expression profiles exhibited at both regional and subcellularlevels, are inconsistent with the notion that they are transcriptional noise or artifacts of chromatin remodeling. Our results show that the majority of ncRNAsare expressed in the brain and provide strong evidence that the majority of processed transcripts with no protein-coding capacity function intrinsically as RNAs. 哺乳动物转录组中的一个主要部分包含长非编码RNA，它们没有或很少有蛋白编码能力。只有一部分被发现并进行了深入的研究，大部分还未知。 艾伦脑图谱利用原位杂交，在成年小鼠大脑中发现了849ncRNA的表达，并且绝大多数与特异的神经解剖区域，细胞类型或亚细胞定位相关。 比较ncRNA和与它们相关的蛋白编码基因揭示了两者间的复杂关系，与在区域和亚细胞水平的特异表达结合，说明这些ncRNA并不是噪音或者染色质重塑的加工品。 我们的结果表明ncRNA在大脑中的表达为不具蛋白编码能力的转录物作为RNA产生功能提供了强有力的证据。 更多资料请登录www.bioknow.cn |
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