刚试用过,例如:
名称:共表达RSV中和抗原的DNA和非复制型腺病毒重组体的构建及其保护作用
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中文摘要: RSV是导致婴幼儿严重下呼吸道感染的最重要的病毒病原,同时也是引起老人发病和死亡的重要原因。病毒感染机体后的免疫保护机制尚未明确,也无特异性防治方法。以真核表达质粒和非复制型腺病毒为载体共表达RSV两种中和抗原F和G,并研究其免疫保护作用是探索RSV疫苗的有益尝试。目前,已经完成研究计划中的一些重要内容,如两种RSV中和抗原编码基因的克隆,重组腺病毒简便高效的改建方法,可进行共表达的腺病毒载体系统的构建,分别表达F和G蛋白的非复制型腺病毒重组体的构建和鉴定工作等。已培养硕士研究生3名,发表论文4篇,其中核心期刊3篇。这些工作为继续完成余下的课题任务奠定了基础。
中文主题词: 呼吸道合胞病毒;腺病毒重组体;免疫保护作用;中和抗原;共表达
英文摘要: Human respiratory syncytial virus (RSV) is the most important viral etiologic agent of pediatric lower respiratory tract disease worldwide. RSV disease is also responsible for considerable morbidity and mortality in the elderly. The mechanisms that contribute to the disease protection are not fully understood. Currently no safe and efficacious RSV vaccine is available. It will be helpful for the RSV vaccine program to investigate their expression and protective immunity of DNA and replication-defective adenoviral recombinants encoding the attachment (G) and fusion (F) protein. Till now, we have finished the clone and eukaryotic expression of F and G gene of subgroup A Long strain RSV, established a more effective and easier method for constructing recombinant adenovirus based on AdEasy System, constructed replication deficient adenovirus vector system used as expressing F and G protein simultaneously and the adenoviral recombinants expressing the F and G protein, respectively. All these achievements provide a good basis for further study.
英文主题词: respiratory syncytial virus;protective immunity;adenovirus recombinant;neutralization antigen;co-expression