In 500 ml dH2O:
Monobasic sodium phosphate 1.8 g
Dibasic sodium phosphate 23.25 g
NaCl 5 g
pH to desired value then add dH2O to 1L
Maximum shelf life after receipt: 1 month
The pH of this buffer is 7.4. this buffer is hypertonic to most body fluids. Millonig recommended to use this buffer for the fixation of very hydrated tissues with osmium tetroxide. For marine organisms, a higher concentration of sodium chloride (3%) is recommended.
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