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1.Expression and purification of recombinant rat full-length HMGB1 protein (HMG = high mobil- ity group) and its domains A (residues 1¨C84 andB(residues 85¨C180 ) (HMGB1a and HMGB1b, respectively) were carried out as described 2¡¢The EcoRI/PvuII restriction fragment (92 bp) from pUC19 plasmid was modified with cisplatin or complexes 1 or 2 to desired rbvalue in 0.01 M NaClO4 at 37 8C, in dark for 24 h. |
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Mally89(½ð±Ò+2, ·ÒëEPI+1): ´úlz·¢·Å£¡~ 2011-04-27 00:14:27
Mally89(½ð±Ò+2, ·ÒëEPI+1): ´úlz·¢·Å£¡~ 2011-04-27 00:14:27
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