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Test 1 Medium: pH 6.5 phosphate buffer with 1% sodium lauryl sulfate (Transfer 206 mL of 1 M monobasic sodium phosphate monohydrate, 196 mL of 0.5 M anhydrous dibasic sodium phosphate, and 50.0 g of sodium lauryl sulfate to a 5000-mL volumetric flask. Add about 4000 mL of water, and mix well. If necessary, adjust with 1 N sodium hydroxide to a pH of 6.5. Dilute with water to volume.); 500 mL Test 3 Medium: pH 6.5 phosphate buffer with 1% sodium lauryl sulfate (5.7 g/L of monobasic sodium phosphate monohydrate, 2.8 g/L of dibasic sodium phosphate, and 10 g/L of sodium lauryl sulfate in water, adjusted, if necessary, with diluted sodium hydroxide to a pH of 6.5 ± 0.05); 500 mL rt [ Last edited by shixiu on 2010-12-14 at 10:51 ] |
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