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dianshineng

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[交流] 请帮忙翻译一个关于口蹄疫病毒的英文摘要

A nucleotides fragment 2020VP1 related to T-cell epitope (aa21-40) and B-cell epitope (aa141-160) of VP1 of Food-and-mouth disease virus (FMDV)type O was synthesized, and the recombined expression vector r2020-LTB-2020-STI was correctly constructed, which was used to express the fusion protein consisted of two copies of aa21-40aa141-160 of FMDV VP1 and LTB and STI Enterotoxins of Escherichia coli. The fusion protein with molecular weight of about 45kD was successfully expressed in E.coli BL21(DE3) RIL at high level and confirmed by SDS-PAGE. The purified fusion protein could be specifically recognized by CTB antibody. The purified protein was then used to vaccinate guinea pigs, rabbits and Balb/c mice at 6-8 week age, and immune responses were finally observed. The fusion protein could induce proliferation of spleen T cells in vaccinated guinea pigs and elicit a high level of neutralizing antibody in rabbits. It could be concluded that the fusion protein could activate FMDV-specific cellular immune-response and humoral immune-response simultaneously. At the same time, the vaccinated mice could survive challenge of Enterotoxigenic Escherichia coli C83902, and the sera of vaccinated rabbits could neutralize the STI toxin. Moreover, the fusion protein had no STI toxicity, indicating that the fusion protein had LTB and STI immunogenicities. Therefore, it can be concluded that the fusion protein could be explored as a potentially efficient FMDV and ETEC vaccine.

谢谢翻译帝们哈

[ Last edited by dianshineng on 2010-12-8 at 18:13 ]

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cfm877

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dianshineng(金币+50, 翻译EPI+1): 2010-12-09 00:01:45

合成了与FMDV病毒O型的VPI的T细胞单抗原决定簇（aa21-40）和B细胞单抗原决定簇相关的核苷酸片段，并正确构建了重组表达载体 r2020-LTB-2020-STI ，它用来表达由两套FMDV VP1和LTB 的 aa21-40aa141-160 和大肠杆菌的STI肠毒素构成的融合蛋白。分子量约为45kD的融合蛋白在大肠杆菌E.coli BL21(DE3) RIL中成功高水平表达并通过SDS - PAGE验证。纯化的融合蛋白能被CTB抗体特异识别。该融合蛋白能够诱导免疫豚鼠的脾T细胞增殖，并在兔子中诱导出高水平的中和抗体。可以得出这样的结论：该融合蛋白能激活口蹄疫病毒特异性细胞免疫反应和体液免疫反应。同时，接种疫苗的老鼠在大肠杆菌肠毒素的挑战下可以存活下来，接种疫苗的兔子的血清可中和STI毒素。