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小木虫论坛-学术科研互动平台 » 生物医药区 » 分子生物 » 综合其他 » 【求助/交流】真核细胞到底是单顺反子还是多顺反子？？？
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njym08

木虫 (正式写手)

[交流] 【求助/交流】真核细胞到底是单顺反子还是多顺反子？？？

rt，最近在看生物化学，发现这本书前后写的不一样。
前面讲原核和真核细胞mRNA区别的时候说：原核是单顺反子，真核是多顺反子。
后面讲原核和真核蛋白质合成差别的时候说：真核mRNA是多顺反子

我凌乱了，记得以前看这本书第四版的时候就发现这个问题了，如今看第六版，一样的问题还在，也不知修订一下~~百度上说法各异，不可取信

请教各位大虾：真核到底是单顺反子，还是多顺反子？？？？望权威解答！！！
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1楼 2010-12-08 00:24:26
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wangleisdnu

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njym08(金币+1): 2010-12-08 09:22:34
Polycistronic mRNA is a mRNA that encodes several proteins and is characteristic of many bacterial and chloroplast mRNAs. Polycistronic mRNAs consist of a leader sequence which precedes the first gene. The gene is followed by an intercistronic region and then another gene. A trailer sequence follows the last gene in the mRNA. Examples of a polycistronic transcripts are found in the chloroplast. One region that exhibits a group of different polycistronic messages from the same region is the psbb/psbH/petB/petD region. The following table lists the genes, their products and the complex of which the product is a part.
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2楼2010-12-08 04:18:01
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wangleisdnu

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njym08(金币+1): 2010-12-08 09:22:41
Monocistronic mRNA is a mRNA that encodes only one protein and all eukaryotic mRNAs are monocistronic. The development of the mature monocistronic eukaryotic transcript involves several different processing steps. These steps are:

   1. 5' capping
   2. 3' polyadenylation
   3. splicing together of exons if introns are present

Each of these steps are post-transcriptional modification steps. Thus the original transcript is not the same as the final product. All of the post-transcriptional steps occur in the nucleus of the cell and the resultant product, the mRNA, is transported to the cytoplasm for translation. So what is the original product of transcription? This product is called hnRNA. This stands for heterogeneous nuclear RNA. Not all of the hnRNA becomes a mRNA product. One question that is yet unresolved is how control is exerted over gene expression? Can control be at the steps which mature the hnRNA? How is transcription of tissue specific genes regulated? These are important question that are currently being researched.

hnRNA transcription is only a small portion of the transcription which occurs in the nucleus. Up to 90% of the transcription is for the production of rRNA. But if we limit our discussion to hnRNA some important observations can be made. First, the complexity of the hnRNA is 4 to 10 times greater than that of the mRNA. This suggest two possibilities. One, we already know and that is that splicing of genes reduces the size of the final mRNA product in comparison to the original transcript. But this does not account completely for the differences in complexity. The majority of the difference appears to be accounted for by destruction of hnRNAs before they develop into mature mRNAs. hnRNAs are a broad class of products:

    * Average size - 8000 - 10,000 bases
    * Range - 2000 - 14,000 bases

Since the average mRNA size is 1800-2000 bases, a considerable amount of trimming must occur.

hnRNA does not actually exist in the nucleus as naked RNA but is actually associated with protein. This complex is designated hnRNP and is called heteronuclear ribonuclearprotein. hnRNP is associated with the nuclear matrix. Since chromatin is attached to the nuclear matrix it seems logical that the primary transcript will also become attached to the matrix just after transcription.

The lifetime of hnRNA is quite short usually from one minute to one hour at the most. So what occurs? The first step in processing is the addition of a Cap. The cap is a 5' methyl guanosine that is added immediately after the start of transcription. Capping occurs so quickly that we rarely see the original 5' base of the message. The linkage between the 5' methyl guanosine is not the typical 5'-3' linkage but is a 5'-5' linkage. The reaction is catalyzed by the enzyme guanylyl transferase. The guanosine that is added is always methylated at the 7 position of the guanine base (7mG). This is called cap 0. In addition a methyl group is added to 2'-OH of the original base in the mRNA. This is catalyzed by 2'-O-methyl-transferase, and this methyl group is referred to as cap 1. Other methylations can occur, but we won't consider them.

About 25% of hnRNA eventually matures in polyadenylated mRNA. Not all mRNAs are polyadenylated. The histone mRNAs are a notable exception to the rule. If an inhibitor of polyadenylation is added to a reaction hnRNA is not converted into mRNA. Thus polyadenlyation is a requirement for mRNA to appear.

Termination of transcription is not understood at all. One sequence that is invariant in eukaryotic mRNA is the sequence 5'-AAUAAA-3' that is found about 10-30 bp upstream of the poly A tail. The question that this raises is whether this sequence is required for polyadenylation. Deletions or mutations of this sequence will eliminate polyadenylation. But surprising, point mutation greatly reduce the number of molecules that are cleaved. But those that are cleaved are polyadenylation. Thus, this sequence seems to be required for cleavage of the primary transcript. The poly-A tail is added by the enzyme poly(A) polymerase.
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3楼2010-12-08 04:18:56
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njym08

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all eukaryotic mRNAs are monocistronic
多谢楼上的，还有个疑问
这本书里说真核细胞蛋白质合成与原核细胞差别的时候，其中一条说“真核细胞的mRNA是单顺反子，有多个Shine-Dalgarno顺序，有多个起始位点”，根据你的摘录，那前半句肯定是错的了，那“有多个Shine-Dalgarno顺序，有多个起始位点”呢？是对的不？
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4楼2010-12-08 09:22:21
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njym08

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自己顶
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6楼2010-12-08 16:57:58
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ceraccerac5楼
2010-12-08 09:32   回复  
rainwander:求助帖，禁止纯表情回复......你是新虫，就不扣金币了，下次注意啊。哈哈 2010-12-08 10:19:42
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