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【求助】求助翻译 谢谢
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For Coomassie Brilliant Blue detection For Laemmli gels (Figure 2), reconstitute the contents of a vial in 200 μl of a standard 1x sample buffer [0.0625 M Tris-HCl, 2% SDS, 10% (v/v) glycerol (optional), 0.1 M DTT and 0.01% bromophenol blue, pH 6.8]. For PhastGel™, ExcelGel™ and CleanGel™ precast gels, reconstitute the contents of a vial in 200 μl of 10 mM Tris-HCl, 2% SDS, 0.1 M DTT, 0.01% bromophenol blue and 1 mM EDTA, pH 8.0. For silver stain detection For silver staining (Figure 3), reconstitute the contents of a vial as described for Coomassie blue staining, then dilute aliquots by at least 50-fold in 1x sample buffer 上面的是上文，只翻译红色部分 谢谢大家 [ Last edited by wbz5102 on 2010-12-5 at 19:03 ] |
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