| 查看: 1452 | 回复: 4 | |||
[交流]
求高手帮我翻译这一段，有很多专业术语，生命科学这块的虫子应该很熟悉
|
|
Competitive ELISA for quantification of trastuzumab. Tissues from BT474 tumor mice that received trastuzumab injections were homogenized in 1 ml of 0.1 M Glycine pH2 with 1% Tween-20 and protease inhibitors followed by a centrifugation (4˚C, 10 min, 14,000 rpm). Six hundred microliters of supernatant was collected, and added with 150 μl of 1 M Tris pH8 and 22.5 μl of 5 M NaCl to neutralize the pH. Microtiter wells coated with 5 mg/ml rabbit anti-human IgG were incubated with the tissue extracts and 1 μg/ml of biotinylated human IgG. After 2 hours of incubation at room temperature, the wells were washed with PBS containing 0.01% Tween 20, added with streptavidin-conjugated horseradish peroxidase, and incubated for 30 minutes at room temperature. The amount of biotinylated human IgG captured on the microtiter wells was quantified with 2,2-azino-bis(3-etylbenzthiazoline-6-sulfonic acid) as a substrate and the absorbance at 405 nm was measured. To obtain standard curves, the tissue extracts were substituted with various concentrations of trastuzumab (ranging from 0.01 to 10 μg/ml) in the ELISA. |
回复此楼» 猜你喜欢
号外号外
已经有11人回复
-
能否退出参与的面上项目解除限项
已经有24人回复
-
filecode，4个jtjc了
已经有17人回复
-
人气不行了
已经有9人回复
-
没有任何消息-是不是就凉了
已经有6人回复
-
如果此刻你正在为国基感到焦虑，不妨来听听这首《基金之外》
已经有5人回复
-
2026国自然函评费到账
已经有19人回复
-
建议基金发布提前给出明确的时间点
已经有16人回复
-
今日不放榜？网传国自然预计 8 月 27 日可查结果
已经有21人回复
-
估计是周四
已经有4人回复
2楼2010-12-05 19:27:42
3楼2010-12-07 16:03:58
4楼2010-12-07 19:15:08
5楼2010-12-07 19:16:24
20