24小时热门版块排行榜

返回列表

scncwxy

木虫 (著名写手)

应助: 14 (小学生)
金币: 4148.4
帖子: 1414
在线: 473.9小时
虫号: 896916

[交流] 【求助】关于道尔顿单位换算的问题

我看到一篇control release上文献说投放5克透明质酸（MW132k,12.5mmol）进行反应，这里132k指的是132k道尔顿吧，也就等于132000g/molX12.5mmol=1650g 这个也太多了吧，前面的不是5g吗？本人新手，谁能帮个忙啊！！

原文是这样的2.2.1. Synthesis of thiol functionalized HA (HA-SH)
Five grams of HA (MW 132K, 12.5 mmol) was dissolved in 250 ml of PBS solution (pH 7.0) and mixed well until no aggregation was observed. Three molar excess amounts of EDC (37.5 mmol) and HOBt (37.5 mmol) were added and stirred for 2 h, and then cystamine dihydrochloride (37.5 mmol) was added dropwise and mixed overnight. The reaction mixture was dialyzed for 24 h to remove unreacted cystamine and EDC and HOBt coupling agents (MW cut-off: 3500). Cystamine conjugated HA was treated with five molar excess of DTT and stirred for 24 h to cleave the disulfide bond in the conjugated cystamine. Thiol functionalized HA (HA-SH) was dialyzed against deionized water at pH 3.0 for 2 days (MW cut-off: 20,000). The final product was lyophilized for 3 days and stored at −20 °C until use. Percent thiol modification was determined by the Elman's assay using L-cysteine as a standard. Rhodamine labeled HA-SH (MW 17K) was also prepared by conjugating an amine-reactive rhodamine dye to HA using the same conjugation chemistry as above.

[ Last edited by scncwxy on 2010-11-29 at 16:52 ]

回复此楼

» 猜你喜欢

写了一篇“相变储能技术在冷库中应用”的论文，论文内容以实验为主，投什么期刊合适？已经有3人回复
需要合成515-64-0，50g，能接单的留言已经有3人回复
最近几年招的学生写论文不引自己组发的文章已经有10人回复
中科院杭州医学所招收博士生一名（生物分析化学、药物递送）已经有3人回复
临港实验室与上科大联培博士招生1名已经有8人回复
26申博自荐已经有7人回复
想换工作。大多数高校都是评职称时认可5年内在原单位取得的成果吗？已经有4人回复
带资进组求博导收留已经有9人回复

» 抢金币啦！回帖就可以得到:

查看全部散金贴

1楼 2010-11-29 15:35:05

已阅回复此楼关注TA 给TA发消息送TA红花 TA的回帖

gjapplefly

铁杆木虫 (正式写手)

应助: 13 (小学生)
金币: 6004.6
帖子: 602
在线: 275.6小时
虫号: 1060103

★
scncwxy(金币+1):谢谢参与
scncwxy(金币+19): 2010-12-02 11:01:01

道尔顿和分子质量同数不同单位，思来想去，觉得只可能是文献中有错，呵呵

[ Last edited by gjapplefly on 2010-12-5 at 11:27 ]

赞一下(1人)

回复此楼

2楼2010-12-02 09:34:25

已阅回复此楼关注TA 给TA发消息送TA红花 TA的回帖

scncwxy

木虫 (著名写手)

应助: 14 (小学生)
金币: 4148.4
帖子: 1414
在线: 473.9小时
虫号: 896916

引用回帖:

Originally posted by gjapplefly at 2010-12-02 09:34:25:
把文章题目贴上来，找找原文看看

Target-specific intracellular delivery of siRNA using degradable hyaluronic acid nanogels
J Control Release. 2007 Jun 4;119(2):245-52. Epub 2007 Feb 27.
里面methods的第一段开头就是

赞一下

回复此楼

3楼2010-12-02 10:57:10

已阅回复此楼关注TA 给TA发消息送TA红花 TA的回帖