24小时热门版块排行榜

返回列表

scncwxy

木虫 (著名写手)

应助: 14 (小学生)
金币: 4140.4
帖子: 1414
在线: 473.9小时
虫号: 896916

[交流] 【求助】关于道尔顿单位换算的问题

我看到一篇control release上文献说投放5克透明质酸（MW132k,12.5mmol）进行反应，这里132k指的是132k道尔顿吧，也就等于132000g/molX12.5mmol=1650g 这个也太多了吧，前面的不是5g吗？本人新手，谁能帮个忙啊！！

原文是这样的2.2.1. Synthesis of thiol functionalized HA (HA-SH)
Five grams of HA (MW 132K, 12.5 mmol) was dissolved in 250 ml of PBS solution (pH 7.0) and mixed well until no aggregation was observed. Three molar excess amounts of EDC (37.5 mmol) and HOBt (37.5 mmol) were added and stirred for 2 h, and then cystamine dihydrochloride (37.5 mmol) was added dropwise and mixed overnight. The reaction mixture was dialyzed for 24 h to remove unreacted cystamine and EDC and HOBt coupling agents (MW cut-off: 3500). Cystamine conjugated HA was treated with five molar excess of DTT and stirred for 24 h to cleave the disulfide bond in the conjugated cystamine. Thiol functionalized HA (HA-SH) was dialyzed against deionized water at pH 3.0 for 2 days (MW cut-off: 20,000). The final product was lyophilized for 3 days and stored at −20 °C until use. Percent thiol modification was determined by the Elman's assay using L-cysteine as a standard. Rhodamine labeled HA-SH (MW 17K) was also prepared by conjugating an amine-reactive rhodamine dye to HA using the same conjugation chemistry as above.

[ Last edited by scncwxy on 2010-11-29 at 16:52 ]

回复此楼

» 猜你喜欢

有时候真觉得大城市人没有县城人甚至个体户幸福已经有11人回复
表哥与省会女结婚，父母去帮带孩子被省会女气回家生重病了已经有7人回复
同年申请2项不同项目，第1个项目里不写第2个项目的信息，可以吗已经有8人回复
依托企业入选了国家启明计划青年人才。有无高校可以引进的。已经有7人回复
依托企业入选了国家启明计划青年人才。有无高校可以引进的。已经有10人回复
天津大学招2026.09的博士生，欢迎大家推荐交流（博导是本人）已经有9人回复
有院领导为了换新车，用横向课题经费买了俩车已经有10人回复
AI 太可怕了，写基金时，提出想法，直接生成的文字比自己想得深远，还有科学性已经有6人回复

» 抢金币啦！回帖就可以得到:

查看全部散金贴

1楼 2010-11-29 15:35:05

已阅回复此楼关注TA 给TA发消息送TA红花 TA的回帖

gjapplefly

铁杆木虫 (正式写手)

应助: 13 (小学生)
金币: 6004.6
帖子: 602
在线: 275.6小时
虫号: 1060103

★
scncwxy(金币+1):谢谢参与
scncwxy(金币+19): 2010-12-02 11:01:01

道尔顿和分子质量同数不同单位，思来想去，觉得只可能是文献中有错，呵呵

[ Last edited by gjapplefly on 2010-12-5 at 11:27 ]

赞一下(1人)

回复此楼

2楼2010-12-02 09:34:25

已阅回复此楼关注TA 给TA发消息送TA红花 TA的回帖

scncwxy

木虫 (著名写手)

应助: 14 (小学生)
金币: 4140.4
帖子: 1414
在线: 473.9小时
虫号: 896916

引用回帖:

Originally posted by gjapplefly at 2010-12-02 09:34:25:
把文章题目贴上来，找找原文看看

Target-specific intracellular delivery of siRNA using degradable hyaluronic acid nanogels
J Control Release. 2007 Jun 4;119(2):245-52. Epub 2007 Feb 27.
里面methods的第一段开头就是

赞一下

回复此楼

3楼2010-12-02 10:57:10

已阅回复此楼关注TA 给TA发消息送TA红花 TA的回帖