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题目：Synthesis of γ-aminobutyric acid (GABA) by Lactobacillus plantarum DSM19463: functional grape must beverage and dermatological applications 摘要：Agriculture surplus were used as substrates to synthesize γ-aminobutyric acid (GABA) by Lactobacillus plantarum DSM19463 for the manufacture of a functional beverage or as a novel application for dermatological purposes. Dilution of the grape must to 1 or 4% (w/v) of total carbohydrates favored higher cell yield and synthesis of GABA with respect to whey milk. Optimal conditions for synthesizing GABA in grape must were: initial pH 6.0, initial cell density of Log 7.0 cfu/mL, and addition of 18.4 mM L-glutamate. L. plantarum DSM19463 synthesized 4.83 mM of GABA during fermentation at 30°C for 72 h. The fermented grape must also contain various levels of niacin, free minerals, and polyphenols, and Log 10.0 cfu/g of viable cells of L. plantarum DSM19463. Freeze dried preparation of grape must was applied to the SkinEthic® Reconstructed Human Epidermis or multi-layer human skin model (FT-skin tissue). The effect on transcriptional regulation of human beta-defensin-2 (HBD-2), hyaluronan synthase (HAS1), filaggrin (FGR), and involucrin genes was assayed through RT-PCR. Compared to GABA used as pure chemical compound, the up-regulation HBD-2 was similar while the effect on the expression of HAS1 and FGR genes was higher. |
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xiaoyi_yeah(金币+10, 翻译EPI+1): 2010-11-28 23:25:57
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题目：：DSM19463乳酸乳杆菌合成的γ-氨基丁酸可以生产功能性葡萄汁饮料，也可用于皮肤疾病的治疗 摘要：农业产品葡萄的剩余，可作为DSM19463乳酸乳杆菌合成γ-氨基丁酸的底物以生产一种功能饮料来使用，或者被新颖的用于治疗皮肤科的疾病。该葡萄汁必须被稀释到总的碳水化合物的含量 1 or 4%(w/v)，γ-氨基丁酸关于乳清蛋白的合成。在葡萄汁里合成γ-氨基丁酸最优的条件是：初始的ph值为6，细胞浓度为Log7.0 cfu/mL,再添加18.4 mM L-谷氨酸。DSM19463乳酸乳杆菌 30°C 条件下发酵72小时可合成4.83 mM γ-氨基丁酸，已经发酵的葡萄汁也含有不同含量的烟酸、游离的矿物质、多酚以及含量为Log 10.0 cfu/g 的DSM19463乳酸乳杆菌的活细胞。冻干的葡萄汁也应用与人类再生上皮细胞以及人类的皮肤模型，人体防御素-2、透明质酸、丝聚蛋白以及包蛋白基因对转录的调节已经通过反转录酶-聚合链酶实验得到验证。它作为单纯的化合物，在上调人体防御素-2的作用上，冻干的葡萄汁跟γ-氨基丁酸比是相似的，然而在 HAS1 and FGR基因的表达上的作用上它则显得更强。 |
2楼2010-11-20 11:39:58
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