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【通告】高手指点:乙二胺调节pH值时,流动相的颜色变了,这是为什么???
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Sodium perchlorate buffer 0.1 M was prepared in deionized distilled water. The mobile phase consisted of buffer:ethanol:diethylamine (70:30:0.5% v/v) with a 35.24 mM concentration of β-CD. The final pH of the mobile phase was adjusted to 6.4。 配好缓冲溶液并与乙醇混合以后,再加乙二胺调节pH时,只要乙二胺稍过量流动相的颜色就会变成黄色,这个怎么回事,高手们解释一下 |










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