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【答案】应助回帖
whj8897(金币+1, 博学EPI+1): 2010-09-24 21:48:45
This generated strains of nisin to optimize the fermentation medium. First, using Plackett - Burman (PB) experimental design method, yeast extract, glucose, KH2PO4, MgSO4, Tween80, fermentation time, pH, fermentation temperature, inoculum size and other factors 9 studied the effects of nisin, selected three major factors: glucose, Tween80, pH; then steepest ascent, central composite design (CCD) and response surface methods (RSM) to determine the optimal concentration of the main factors were: glucose 2.32 g / L, Tween80 0.1 g / L, pH 7.0. Nisin in antimicrobial activity under optimal conditions enhanced the formation of inhibition zone diameter was 24.76 mm, is 1.25 times before optimization.
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