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| a) treating the fermentation broth, extracts or solutions containing rapamycin with water immiscible solvent and concentration; b) addition of a water miscible solvent to effect separation of impurities present; c) optionally, binding of the solvent containing the product from step (b) to an inert solid, washing the solid with a base and acid, followed by elution; d) subjecting the elute from step (c) or the solvent containing the product from step (b) to silica gel chromatography; e) crystallization of the product obtained from step (d); f) subjecting a solution of the product from step (e) to hydrophobic interaction or reversed phase chromatography; and g) re-crystallization to afford rapamycin in substantially pure form. |
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