| 查看: 216 | 回复: 0 | ||
[资源]
【分享】Immunoelectron Microscopy: Methods and Protocols (657)
|
|
Immunoelectron Microscopy: Methods and Protocols (Methods in Molecular Biology 657) by: Steven D. Schwartzbach, Tetsuaki Osafune en •Publisher: Humana Press •Number Of Pages: 352 •Publication Date: 2010-07-01 •ISBN-10 / ASIN: 160761782X •ISBN-13 / EAN: 9781607617822 Product Description: Immunoelectron microscopy is a key technique that bridges the information gap between biochemistry, molecular biology, and ultrastructural studies placing macromolecular functions within a cellular context. In Immunoelectron Microscopy: Methods and Protocols, expert researchers combine the tools of the molecular biologist with those of the microscopist. From the molecular biology toolbox, this volume presents methods for antigen production by protein expression in bacterial cells, methods for epitope tagged protein expression in plant and animal cells allowing protein localization in the absence of protein specific antibodies as well as methods for the production of anti-peptide, monoclonal, and polyclonal antibodies. From the microscopy toolbox, sample preparation methods for cells, plant, and animal tissue are presented. Both cryo-methods, which have the advantage of retaining protein antigenicity at the expense of ultrastructural integrity, as well as chemical fixation methods that maintain structural integrity while sacrificing protein antigenicity have been included, with chapters examining various aspects of immunogold labeling. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and essential, Immunoelectron Microscopy: Methods and Protocols seeks to facilitate an increased understanding of structure function relationships. http://ifile.it/udtm5v7/160761782X.rar |
回复此楼» 猜你喜欢
调剂求收留
已经有27人回复
-
0831一轮调剂失败求助
已经有9人回复
-
材料相关专业344求调剂双非工科学校或课题组
已经有7人回复
-
求调剂
已经有17人回复
-
322求调剂
已经有6人回复
-
一志愿浙大生物325分求调剂
已经有4人回复
-
本科南方医科大学 一志愿985 药学学硕284分 求调剂
已经有5人回复
-
295分求调剂
已经有12人回复
-
272分材料子求调剂
已经有49人回复
-
211本科材料化工求调剂
已经有16人回复
10