| 查看: 214 | 回复: 0 | ||
[资源]
【分享】Immunoelectron Microscopy: Methods and Protocols (657)
|
|
Immunoelectron Microscopy: Methods and Protocols (Methods in Molecular Biology 657) by: Steven D. Schwartzbach, Tetsuaki Osafune en •Publisher: Humana Press •Number Of Pages: 352 •Publication Date: 2010-07-01 •ISBN-10 / ASIN: 160761782X •ISBN-13 / EAN: 9781607617822 Product Description: Immunoelectron microscopy is a key technique that bridges the information gap between biochemistry, molecular biology, and ultrastructural studies placing macromolecular functions within a cellular context. In Immunoelectron Microscopy: Methods and Protocols, expert researchers combine the tools of the molecular biologist with those of the microscopist. From the molecular biology toolbox, this volume presents methods for antigen production by protein expression in bacterial cells, methods for epitope tagged protein expression in plant and animal cells allowing protein localization in the absence of protein specific antibodies as well as methods for the production of anti-peptide, monoclonal, and polyclonal antibodies. From the microscopy toolbox, sample preparation methods for cells, plant, and animal tissue are presented. Both cryo-methods, which have the advantage of retaining protein antigenicity at the expense of ultrastructural integrity, as well as chemical fixation methods that maintain structural integrity while sacrificing protein antigenicity have been included, with chapters examining various aspects of immunogold labeling. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and essential, Immunoelectron Microscopy: Methods and Protocols seeks to facilitate an increased understanding of structure function relationships. http://ifile.it/udtm5v7/160761782X.rar |
回复此楼» 猜你喜欢
什么是人一生最重要的？
已经有4人回复
-
版面费该交吗
已经有3人回复
-
过年走亲戚时感受到了所开私家车的鄙视链
已经有10人回复
-
今年春晚有几个节目很不错，点赞！
已经有12人回复
-
体制内长辈说体制内绝大部分一辈子在底层，如同你们一样大部分普通教师忙且收入低
已经有12人回复
-
基金正文30页指的是报告正文还是整个申请书
已经有5人回复