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【分享】DNA Vaccines: Methods and Protocols 已有4人参与
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DNA Vaccines: Methods and Protocols. Methods in Molecular Medicine, Vol. 29 简介： This is one of the many Humana Press volumes devoted to 'methods in molecular medicine' and follows the usual format for books in this series. It comprises more than 40 short chapters, each dealing with specific practical aspects of DNA vaccination and providing detailed experimental protocols. It is little more than 10 years since the concept of naked DNA vaccination burst upon the scientific community and many of the contributors to this volume were intimately involved in the early discovery stages of the science. DNA vaccination has yet to fulfil some of the extravagant hopes that were expressed initially and, as is so often the case, it is far more complex than originally thought. Work on the development of DNA vaccines continues apace for a huge range of potential applications and this book will be invaluable for any laboratory involved in research in this field. 评论： "...does an excellent job of describing the current methodologies in their development, what clinical benefit can be expected, and what is known of their mode of action...has thoroughly covered the current state of the art." -Antiviral Research "The present book contains 41 chapters by experts in the field not only describing the state-of-the-art thinking on each topic, but, more importantly, providing a step-by-step recipe for constructing the cost effective plasmids and measuring host immune responses, both humoral and cellular, as well as all the stops in between including delivery, etc....an excellent roadmap as to how to proceed...This comprehensive collection of chapters is the first of its kind in the rapidly moving field to provide an excellent update of all of the recent advances and, more importantly, the actual technical approaches to developing vaccines. This is a book that should be in everyone's library who is interested in pursing studies employing DNA immunization and for graduate students interested in an up-to-date understanding of DNA vaccination." -FEBS Letters "Its 41 chapters provide a valuable introduction, a series of working protocols, and a set of heavily referenced reviews of state-of-the-art methods in the production, use, and analysis of DNA vaccines. The various chapters are especially fruitful in their presentation of detailed techniques for making buffers and complex biological reagents." -Modern Drug Discovery "Tecular Biology series, consists of 41 well written and referenced chapters dealing with certain aspects of DNA vaccination. The subjects addressed range from large scale plasmid isolation to DNA vaccination and autoimmunity, and illuminate the explosive growth this field has undergone in recent years. A substantial amount of the text concentrates on the practical aspects of processing DNA, quality control and immunological responses....In short, this book is a valuable addition to the library of all people actively involved in the field of DNA vaccination. Researchers will find the detailed protocols and innumerable reference valuable. I would recommend this book to any researcher in this field, if only to finally have a book with well organized and referenced protocols instead of a pile of coffee stained photocopies." -Today's Life Science "The scope of the text is wide and covers virtually all aspects of the subject from the initial plasmid preparation and industrial scale up to the regulatory issues that will probably need to be addressed if such vaccines reach licensing stage." - The Journal of Medical Microbiology "Work on the development of DNA vaccines continues aspace for a huge range of potential applications and this book will be invaluable for any laboratory involved in research in this field." -Microbiology Today 第一次发资源分享帖，有做的不好的地方请多指正。给个评价更好，呵呵。 http://www.box.net/shared/vzs2idjkgo [ Last edited by senoy on 2010-7-6 at 14:51 ] |
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