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[交流] 推荐一个绝好的国外透射电镜学习网站

　 推荐一个绝好的国外电镜网站

　 ------Transmission Electron Microscopy Web Course

http://em-outreach.ucsd.edu/web-course/toc.html

　目录

　 The Microscope

I.A. Principles of the Transmission Electron Microscope (TEM)

I.A.1 Origin of the Transmission Electron Microscope
I.A.2. Comparison Between Electron And Light Microscopy
I.A.3. Photons/Electrons
I.A.4. Optics (Lens Theory)
I.A.5. Electron Optics

I.B. Design of the Electron Microscope

I.B.1. Electron Gun
I.B.2. Condenser Lenses
I.B.3. Lens Aberrations and other non-ideal imaging properties
I.B.4. Objective Lens and Specimen Stage
I.B.5. Projector Lens (TBD)
I.B.6. Camera and Viewing System (TBD)
I.B.7. Vacuum System (TBD)
I.B.8. Electrical System (TBD)

I.C. Contrast and Image Formation

I.C.1. Electron Scattering
I.C.2. Amplitude/Phase Contrast
I.C.3. Phase Contrast Transfer Theory
I.C.4. Multiple Scattering in Thick Specimens
I.C.5. Other Methods in Enhancing Contrast
I.D. Microcope Disturbances and Alignment
I.D.1 Introduction
I.D.2 Alignment of the Microscope
I.D.3. Disturbances to Microscope Performance

I.E. Operation of the Transmission Electron Microscope

I.E.1. Choice of Accelerating Voltage
I.E.2. Choice of Apertures
I.E.3. Specimen Stage/Holder
I.E.4. Choice of Magnification
I.E.5. Focusing
I.E.6. Magnification Calibration
I.E.7. Resolution Tests
I.E.8. Microscope Maintenance
I.E.9. Microscope Maintenance
I.E.10. Photography

I.F. Other Modes of Transmission Electron Microscope Operation

I.F.1. Electron Diffraction
I.F.2. Dark Field Microscopy
I.F.3. High Resolution Transmission Electron Microscopy
I.F.4. Tilting and Stereo Microscopy
I.F.5. Low Temperature
I.F.6. Energy Loss
I.F.7. X-ray Microanalysis

　 The Specimen

II.A. Biological Specimen Preparation Techniques

II.A.1. Support Films
II.A.2. Thin Sectioning (Fixation/Dehydration/Embedding/Staining)
II.A.3. Negative Staining
II.A.4. Metal Shadowing
II.A.5. Unstained Specimens
II.A.6. Freeze Drying/Etching/Fracture
II.A.7. Autoradiography

II.B. Radiation Effects

II.B.1. Introduction
II.B.2. Dose/Dose Rate
II.B.3. Primary Effects of Radiation Damage to Biological Specimens
II.B.4. Secondary Effects of Radiation Damage
II.B.5. Ways to Measure Damage/Critical Dose
II.B.6. Procedures to Reduce Radiation Damage
II.B.7. Relation betwen Contrast, Resolution and Radiation Damage
II.B.8. Radiation Effects in Negatively-Stained Specimens
II.B.9. Radiation Effects in Frozen-Hydrated Specimens

　 The Micrograph

III.A. Introduction to Image Analysis and Processing of Biological Specimens

III.A.1. Image Processing Techniques: Real and Reciprocal Space Methods
III.A.2. Fourier Image Processing
III.A.3. Periodic/Non-Periodic Specimens
III.A.4. Applications and Advantages of Image Processing

III.B. Sources of Noise in TEM Images of Biological Specimens

III.B.1. Specimen Support Film
III.B.2. Specimen
III.B.3. Microscope
III.B.4. Photography

III.C. Crystals, Symmetry, and Diffraction

III.C.1. Definition of Terms
III.C.2. Crystals
III.C.3. Lattices
III.C.4. Crystal Structure
III.C.5. Symmetry
III.C.6.Diffraction

III.D. Fourier Image Processing Techniques

III.D.1. Optical Diffraction
III.D.2. Optical Filtering
III.D.3. Digital Fourier Analysis of Electron Micrographs

III.E. Analyzing Images of Specimens of Different Types

III.E.1.Planar specimens (two-dimensional sheets)
III.E.2. Three-Dimensional Crystals
III.E.3. Particles with Rotational Symmetry
III.E.4. Helical

III.F. Considerations Concerning Specimen Preparation and Microscopy

III.F.1. Sample Preparation
III.F.2. Imaging Conditions

III.G. Image Analysis Using Real Space and Other Reconstruction Methods

III.H. Interpretation and Display of Image Analysis Results

[ Last edited by 小木虫2005 on 2006-3-25 at 13:47 ]

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