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推荐一个绝好的国外电镜网站 ------Transmission Electron Microscopy Web Course http://em-outreach.ucsd.edu/web-course/toc.html 目录 The Microscope I.A. Principles of the Transmission Electron Microscope (TEM) I.A.1 Origin of the Transmission Electron Microscope I.A.2. Comparison Between Electron And Light Microscopy I.A.3. Photons/Electrons I.A.4. Optics (Lens Theory) I.A.5. Electron Optics I.B. Design of the Electron Microscope I.B.1. Electron Gun I.B.2. Condenser Lenses I.B.3. Lens Aberrations and other non-ideal imaging properties I.B.4. Objective Lens and Specimen Stage I.B.5. Projector Lens (TBD) I.B.6. Camera and Viewing System (TBD) I.B.7. Vacuum System (TBD) I.B.8. Electrical System (TBD) I.C. Contrast and Image Formation I.C.1. Electron Scattering I.C.2. Amplitude/Phase Contrast I.C.3. Phase Contrast Transfer Theory I.C.4. Multiple Scattering in Thick Specimens I.C.5. Other Methods in Enhancing Contrast I.D. Microcope Disturbances and Alignment I.D.1 Introduction I.D.2 Alignment of the Microscope I.D.3. Disturbances to Microscope Performance I.E. Operation of the Transmission Electron Microscope I.E.1. Choice of Accelerating Voltage I.E.2. Choice of Apertures I.E.3. Specimen Stage/Holder I.E.4. Choice of Magnification I.E.5. Focusing I.E.6. Magnification Calibration I.E.7. Resolution Tests I.E.8. Microscope Maintenance I.E.9. Microscope Maintenance I.E.10. Photography I.F. Other Modes of Transmission Electron Microscope Operation I.F.1. Electron Diffraction I.F.2. Dark Field Microscopy I.F.3. High Resolution Transmission Electron Microscopy I.F.4. Tilting and Stereo Microscopy I.F.5. Low Temperature I.F.6. Energy Loss I.F.7. X-ray Microanalysis The Specimen II.A. Biological Specimen Preparation Techniques II.A.1. Support Films II.A.2. Thin Sectioning (Fixation/Dehydration/Embedding/Staining) II.A.3. Negative Staining II.A.4. Metal Shadowing II.A.5. Unstained Specimens II.A.6. Freeze Drying/Etching/Fracture II.A.7. Autoradiography II.B. Radiation Effects II.B.1. Introduction II.B.2. Dose/Dose Rate II.B.3. Primary Effects of Radiation Damage to Biological Specimens II.B.4. Secondary Effects of Radiation Damage II.B.5. Ways to Measure Damage/Critical Dose II.B.6. Procedures to Reduce Radiation Damage II.B.7. Relation betwen Contrast, Resolution and Radiation Damage II.B.8. Radiation Effects in Negatively-Stained Specimens II.B.9. Radiation Effects in Frozen-Hydrated Specimens The Micrograph III.A. Introduction to Image Analysis and Processing of Biological Specimens III.A.1. Image Processing Techniques: Real and Reciprocal Space Methods III.A.2. Fourier Image Processing III.A.3. Periodic/Non-Periodic Specimens III.A.4. Applications and Advantages of Image Processing III.B. Sources of Noise in TEM Images of Biological Specimens III.B.1. Specimen Support Film III.B.2. Specimen III.B.3. Microscope III.B.4. Photography III.C. Crystals, Symmetry, and Diffraction III.C.1. Definition of Terms III.C.2. Crystals III.C.3. Lattices III.C.4. Crystal Structure III.C.5. Symmetry III.C.6.Diffraction III.D. Fourier Image Processing Techniques III.D.1. Optical Diffraction III.D.2. Optical Filtering III.D.3. Digital Fourier Analysis of Electron Micrographs III.E. Analyzing Images of Specimens of Different Types III.E.1.Planar specimens (two-dimensional sheets) III.E.2. Three-Dimensional Crystals III.E.3. Particles with Rotational Symmetry III.E.4. Helical III.F. Considerations Concerning Specimen Preparation and Microscopy III.F.1. Sample Preparation III.F.2. Imaging Conditions III.G. Image Analysis Using Real Space and Other Reconstruction Methods III.H. Interpretation and Display of Image Analysis Results [ Last edited by 小木虫2005 on 2006-3-25 at 13:47 ] |
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