| 查看: 447 | 回复: 0 | |||
| 当前主题已经存档。 | |||
[交流]
用PHOTOSHOP将两种荧光图象重叠
|
|||
|
利用PHOTOSHOP将两种荧光图象重叠，从而达到激光共聚焦效果。 操作步骤如下： Photoshop Procedure to Colorize Immuno Images Open Photoshop Open both images (Cy3-red and Hoechest33258-blue)— 1. Choose one image Image—Mode—RGB Color Image—Adjust—Brightness/Contrast. Then Save As: 2. Choose other image Image—Mode—RGB Color Image—Adjust—Brightness/Contrast. Then Save As: 3. Select one of the images—(say the Hoechest33258-blue) Go to Channels Click on Red—Go to Image—Adjust—Brightness/Contrast Move both sliders to zero (or -100) Click on Green--Go to Image—Adjust—Brightness/Contrast Move both sliders to zero (or -100) Save 4. Select the other image (Cy3-red) Go to Channels Click on Green—Go to Image—Adjust—Brightness/Contrast Move both sliders to zero (or -100) Click on Blue--Go to Image—Adjust—Brightness/Contrast Move both sliders to zero (or -100) Save 5. Select one of the images Go to Layers Double click on Background—Rename: Cy3-red Save 6. Select the other image Go to Layers Double click on Background—Rename: Hoechest33258-blue Save 7. Select All Copy 8. Go to the other image Paste Change from “Normal” to “Screen” on Layers pallette. Save As |
回复此楼» 猜你喜欢
国自然面上复盘~欢迎讨论
已经有9人回复
-
看板上这么多中的，有点像50人群里49个人都是骗子的那种感觉……
已经有6人回复
-
导师吐槽：我怎么摊上了这么个极品研究生！
已经有6人回复
-
青基如何破局
已经有4人回复
-
基金不中，共勉
已经有8人回复
-
基金未中，这种答复是模板吗？
已经有5人回复
-
为什么资助数各大高校都创新高，自己申请怎么就这么难
已经有5人回复
-
有多少人是今天查系统知道结果的？
已经有17人回复
-
怎么看青基中了没有啊
已经有5人回复
-
梦想
已经有3人回复
投票:
10