| 查看: 431 | 回复: 0 | |||
| 当前主题已经存档。 | |||
[交流]
用PHOTOSHOP将两种荧光图象重叠
|
|||
|
利用PHOTOSHOP将两种荧光图象重叠，从而达到激光共聚焦效果。 操作步骤如下： Photoshop Procedure to Colorize Immuno Images Open Photoshop Open both images (Cy3-red and Hoechest33258-blue)— 1. Choose one image Image—Mode—RGB Color Image—Adjust—Brightness/Contrast. Then Save As: 2. Choose other image Image—Mode—RGB Color Image—Adjust—Brightness/Contrast. Then Save As: 3. Select one of the images—(say the Hoechest33258-blue) Go to Channels Click on Red—Go to Image—Adjust—Brightness/Contrast Move both sliders to zero (or -100) Click on Green--Go to Image—Adjust—Brightness/Contrast Move both sliders to zero (or -100) Save 4. Select the other image (Cy3-red) Go to Channels Click on Green—Go to Image—Adjust—Brightness/Contrast Move both sliders to zero (or -100) Click on Blue--Go to Image—Adjust—Brightness/Contrast Move both sliders to zero (or -100) Save 5. Select one of the images Go to Layers Double click on Background—Rename: Cy3-red Save 6. Select the other image Go to Layers Double click on Background—Rename: Hoechest33258-blue Save 7. Select All Copy 8. Go to the other image Paste Change from “Normal” to “Screen” on Layers pallette. Save As |
回复此楼» 猜你喜欢
体制内长辈说体制内绝大部分一辈子在底层，如同你们一样大部分普通教师忙且收入低
已经有19人回复
-
【博士招生】太原理工大学2026化工博士
已经有8人回复
-
280求调剂
已经有4人回复
-
面上可以超过30页吧？
已经有12人回复
-
什么是人一生最重要的？
已经有6人回复
-
为什么中国大学工科教授们水了那么多所谓的顶会顶刊，但还是做不出宇树机器人？
已经有11人回复
-
网上报道青年教师午睡中猝死、熬夜猝死的越来越多，主要哪些原因引起的？
已经有9人回复
-
版面费该交吗
已经有15人回复