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【求助】有人做量子点显微镜成像的吗？已有5人参与
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显微镜成像的时候有没有发现量子点是一开始激发时不亮，但是激发1分钟后慢慢变得很亮？这种问题怎么解决？ 你们做量子点的时候有没有发现量子点会越照越亮？ |
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8楼2013-10-18 00:08:23
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2楼2010-05-28 12:28:28
3楼2010-05-28 13:57:58
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我做的是普通宽场荧光显微镜，我师兄做的是激光共聚焦，我们俩都观察到这个现象了。 我问了一个老外，在science上发过一篇量子点在活细胞成像的review。他的回答如下： this is not a new phenomenon and has been observed at the very beginning of QD use in biology (more than 10 years ago). The idea is that some of the QDs are dark (this is why the quantum yield (QY) is usually much less than 1, or at least becomes so in real life situations). This is because of surface defects, imperfect coating, etc... However, cells contain a lot of molecules (as you can guess), among which small cystein-rich or thiolated molecules. This group (-SH) is known to passivate QD's surface very well, increasing the QY and in some case suppressing the blinking of individual QDs. The longer the QDs are in cells. the greater this passivation... This is what you are observing. Unfortunately, short of having perfectly passivated QDs from the get-go, you will always observe this in live cells... 他说是表面缺陷导致不亮，但是很多地方都说就是因为表面缺陷导致量子点发光的。 到底是怎么回事？表面缺陷到底是引起量子点不亮还是发亮的原因？ |
4楼2010-05-28 14:56:36