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mofatuzi

新虫 (小有名气)

[交流] 【求助】有人做量子点显微镜成像的吗? 已有5人参与

显微镜成像的时候有没有发现量子点是一开始激发时不亮,但是激发1分钟后慢慢变得很亮?这种问题怎么解决?

你们做量子点的时候有没有发现量子点会越照越亮?
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mofatuzi

新虫 (小有名气)

我现在买的是oceannano的油相QD,然后自己MPA水溶化,oceannano的质量还不错,但是我自己水溶化以后亮度下降很大,结果放在细胞里又慢慢变亮,那我要怎么才能水溶化得比较好啊?
6楼2010-05-30 19:44:57
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eve_css

木虫 (正式写手)

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引用回帖:
Originally posted by mofatuzi at 2010-05-28 10:25:03:
显微镜成像的时候有没有发现量子点是一开始激发时不亮,但是激发1分钟后慢慢变得很亮?这种问题怎么解决?

你们做量子点的时候有没有发现量子点会越照越亮?

您做的是共聚焦成像还是普通的荧光成像呢,好像没发现这种情况呢,一直都很亮。
让金蛋砸死我吧!QQ171394248
2楼2010-05-28 12:28:28
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xiao7

银虫 (正式写手)

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做普通电镜,倒是有放电现象,就是如此。
3楼2010-05-28 13:57:58
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mofatuzi

新虫 (小有名气)

我做的是普通宽场荧光显微镜,我师兄做的是激光共聚焦,我们俩都观察到这个现象了。
我问了一个老外,在science上发过一篇量子点在活细胞成像的review。他的回答如下:
this is not a new phenomenon and has been observed at the very beginning of QD use in biology (more than 10 years ago).
The idea is that some of the QDs are dark (this is why the quantum yield (QY) is usually much less than 1, or at least becomes  so in real life situations). This is because of surface defects, imperfect coating, etc... However, cells contain a lot of molecules (as you can guess), among which small cystein-rich or thiolated molecules. This group (-SH) is known to passivate QD's surface very well, increasing the QY and in some case suppressing the blinking of individual QDs. The longer the QDs are in cells. the greater this passivation... This is what you are observing. Unfortunately, short of having perfectly passivated QDs from the get-go, you will always observe this in live cells...

他说是表面缺陷导致不亮,但是很多地方都说就是因为表面缺陷导致量子点发光的。

到底是怎么回事?表面缺陷到底是引起量子点不亮还是发亮的原因?
4楼2010-05-28 14:56:36
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