应《网络安全法》要求，自2017年10月1日起，未进行实名认证将不得使用互联网跟帖服务。为保障您的帐号能够正常使用，请尽快对帐号进行手机号验证，感谢您的理解与支持！

24小时热门版块排行榜    

小木虫论坛-学术科研互动平台» 学术交流区» 论文翻译 » 论文翻译求助完结子版 » 胰岛素增敏活性筛选实验操作翻译
北京石油化工学院2026年研究生招生接收调剂公告
11/1返回列表
查看: 1280  |  回复: 1
只看楼主 @他人 存档 新回复提醒 (忽略) 收藏    在APP中查看
本帖产生 1 个 翻译EPI ，点击这里进行查看
当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖

jingjing1005

木虫 (小有名气)
★
wypward(金币+1):欢迎新虫，辛苦啦 2010-06-01 10:51:07
hanyan2004(金币+50, 翻译EPI+1): 2010-06-01 23:17:06
hanyan2004(金币+1): 2010-06-02 12:10:53
hanyan2004(金币+1):翻译得很好，谢谢你哈 2010-06-02 21:37:14
Screening test of Insulin-sensitizing activity

3T3-L1 cells were cultured in high glucose DMEM culture meduim with phenol red Containing 10% FBS in six-well plates. When confluence reached 90%, cells were induced to differentiate with a high sugar DMEM culture medium with phenol red containing 10% FBS, Ins, Dex, IBMX. After four days, cells were seeded in 24 well plates after re-digestion, cultured in high glucose DMEM culture medium with phenol red containing 10% FBS for two days. and then they were induced for insulin resistance using high glucose DMEM culture medium with phenol red containing 10% FBS, Ins in lower concentration and Dex in higher concentration. Four days later the cells were transferred to the high glucose DMEM medium without phenol red containing sample, while the control test (normal cells after induction of differentiation) and negative control test (insulin resistant cells after induced differentiation without sample) were established. 10ul supernatants were taken to ELISA plate
after 50hs’ continuous culture to test the glucose level using glucose testing kit and OD values in 490nm. During the experiment, a double recovery was set for each sample hole and the same experiment was repeated twice. The increased sensitivity rate was calculated according to OD values, increased sensitivity rate = [1 - (OD sample-OD blank) / (OD negative-OD blank)] × 100%, the relative increased sensitivity rate = increased sensitivity of sample / increased sensitivity of rosiglitazone.
赞一下(1人) 回复此楼
2楼2010-06-01 10:22:34
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
相关版块跳转 我要订阅楼主 hanyan2004 的主题更新
11/1返回列表
普通表情 龙 兔 虎 猫 高级回复 (可上传附件)
最具人气热帖推荐 [查看全部] 作者 回/看 最后发表
[考研] 288求调剂 一志愿哈工大 材料与化工 +18 洛神哥哥 2026-03-31 18/900 2026-03-31 21:55 by haohaichenji
[考研] 材料调剂 5+6 想要一壶桃花水 2026-03-25 13/650 2026-03-31 21:55 by cqhuanglong
[考研] 一志愿西交大080500材料学硕349 +6 jqx1258 2026-03-31 7/350 2026-03-31 21:08 by yuq
[考研] 346求调剂 一志愿070303有机化学 +11 萝卜炖青菜 2026-03-28 12/600 2026-03-31 17:54 by xhai2011
[考研] 085601 材料工程 313分 求调剂 +6 Ong3 2026-03-27 6/300 2026-03-31 15:18 by yanflower7133
[考研] 生物学 296 求调剂 +7 朵朵- 2026-03-26 9/450 2026-03-31 14:26 by jp9609
[考研] 322求调剂 +10 旧吢 2026-03-24 10/500 2026-03-31 13:31 by 无际的草原
[考研] 085600 295分求调剂 +13 W55j 2026-03-30 15/750 2026-03-31 13:29 by 王亮_大连医科大
[考研] 282求调剂 +3 呼吸都是减肥 2026-03-31 3/150 2026-03-31 10:00 by wangjy2002
[考研] 291求调剂 +12 Y-cap 2026-03-29 15/750 2026-03-31 09:25 by Huaxue_Wang
[考研] 材料与化工调剂一志愿大连海事085600，349 +6 吃的不少 2026-03-30 6/300 2026-03-31 04:17 by fmesaito
[考研] 福建理工大学材料学院先进合金团队招收考研调剂学生 +3 大华金商都 2026-03-30 4/200 2026-03-31 01:04 by 方英俊602
[考研] 285求调剂 +6 AZMK 2026-03-29 9/450 2026-03-30 21:02 by dophin1985
[考研] 324求调剂 +9 hanamiko 2026-03-26 11/550 2026-03-30 14:27 by JourneyLucky
[考研] 材料与化工304求B区调剂 +4 邱gl 2026-03-26 7/350 2026-03-30 08:39 by 探123
[考研] 285求调剂 +4 AZMK 2026-03-27 7/350 2026-03-27 20:59 by AZMK
[考研] 调剂 +3 李嘉图·S·路 2026-03-27 3/150 2026-03-27 11:19 by wangjy2002
[考研] 321求调剂 +6 wasdssaa 2026-03-26 6/300 2026-03-26 20:57 by sanrepian
[考研] 调剂 +4 柚柚yoyo 2026-03-26 4/200 2026-03-26 20:43 by fmesaito
[考研] 303求调剂 +6 蓝山月 2026-03-25 6/300 2026-03-25 22:47 by 418490947
信息提示
关闭
请填处理意见
关闭