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Protein microarray chip with Ni–Co alloy coated surface Most protein microarrays based on the theory of immobilized metal affinity chromatography(IMAC)were fabricated using the chelator or compound of mono-metallic ion,such as Ni2+,to capture the histidine- tagged protein.In this study,a novel protein chip with Ni–Co alloy layer fabricated on the substrate of printed circuit board by electrodeposition was developed.It is an innovative microarray surface with bi- metallic elements,i.e.by means of adding cobalt to enhance the specific binding capability to immobilize functional proteins with His-tag attached.Taguchi method was adopted to determine the optimal elec-trodeposition condition using L18 orthogonal array.Then,the immunoassay was utilized to investigate the properties of the protein chip,and the signal was detected by confocal scanner.The results show that the alloy coating belongs to codeposition of Ni–Co alloy.Due to the affinity adsorption between intermediate metal and protein,this chip provides specific binding.Compared with Ni-coated chip and nitrocellulose(NC)chip,this Ni–Co alloy chip is a high sensitive protein microarray because of high detected fluorescence intensity with low fluorescent background.The proportion of Ni–Co composition will affect the affinity of electroplated layer with proteins and,consequentially,influence the results of immunoassay.Moreover,the test of long-term sensibility of Ni–Co chip showed that it had better binding capability in the first two weeks for coating buffers of pH 7.4 and pH 9 and the immobilization ability began to decay after the third week.The advantages of this novel protein chip include good performance, high repeatability,and inexpensive. |
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看天(金币+1):你保证不是谷歌翻译的？ 2010-07-10 09:27:58
看天(金币+1):你保证不是谷歌翻译的？ 2010-07-10 09:27:58
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蛋白质微阵列芯片镍？CCO的合金涂层表面 大多数蛋白对固定化金属亲和层析理论的基础芯片（IMAC部分）是捏造的使用，如镍离子的螯合剂或单一金属离子，化合物，以捕获组氨酸标签protein.In这项研究中，与镍新型蛋白质芯片？CCO的合金层基板上制作的 印制电路板电镀是developed.It是一个双芯片表面的金属元素的创新，ieby加钴，以提高特异性结合能力，与他的固定标签attached.Taguchi方法手段是通过蛋白质的功能来确定最优球阀+ trodeposition条件下使用正交array.Then 18课，是利用了免疫蛋白芯片调查的性质，信号是由共焦检测scanner.The结果表明，该合金涂层是属于镍共沉积？CCO的alloy.Due中间的金属和蛋白质之间的亲和吸附，此芯片提供特定的镍涂层芯片和binding.Compared 硝酸纤维素（NC）的芯片，这倪？CCO的芯片是一种高合金由于高镍低的检测荧光background.The比例荧光强度敏感蛋白芯片？CCO的成分会影响电镀层与蛋白质的亲和力和，因而，影响的结果 immunoassay.Moreover，在长期的镍敏感性测试？CCO的显示芯片，它在头两个为pH值7.4和pH值9和固定能力缓冲区周使涂层具有约束力的能力开始衰退后，第三week.The这种新型蛋白质芯片的优点包括：良好的表现， 高重复性，又便宜。 |
2楼2010-07-10 09:26:41
