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Enhancement of sensitivity of SPR protein microarray using a novel 3D protein immobilization We discovered a novel method to prepare a protein-based hydrogel,that is,a“Three-Dimensional Nanos-tructured Protein Hydrogel(3D NPH)”,which is composed of protein–polymer hybrid nanoparticles.In this study,we propose a novel protein microarray whose 3D NPH spots were prepared by dispensing a small volume of the solution of protein–polymer mixture on a substrate.The dispensed solution had a short time for cross-linking before its drying-up and the resulting 3D NPH had loosely cross-linked,thin spongy structure.Therefore,the reaction ratio between ligands and analytes was drastically improved in this system compared with the large volume system for Surface Plasmon Resonance(SPR)protein microarray. |
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