| 查看: 331 | 回复: 0 | |||
liushuai3788新虫 (初入文坛)
|
[交流]
【求助/交流】求翻译 An optical PMMA biochip based on fluorescence
|
|
An optical PMMA biochip based on fluorescence anisotropy:Application to C-reactive protein assay An optical platform for point of care testing(POCT)application is developed.The core of the platform is a miniaturised polymethylmetacrylate(PMMA)chip,that consists of two pieces of PMMA that have been custom-machined to produce flow channels,500 m in width and 400 m in height,through which the analysed sample flows.Thanks to the fluorescence anisotropy,a large fraction of the fluorescence emitted by the sensing layer immobilised on the bottom of the PMMA cover travels along the thickness of the PMMA cover itself up to its end-face and it is collected by a plastic optical fibre connected to a photodetector. The potential of the optical chip is investigated with a sandwich assay for the C-reactive protein,one of the markers for inflammatory diseases.The assay covers the working range from 0.1 to 50 mg L?1 with a limit of detection of 0.004 mg L?1.Different combinations of capture and target antibodies were carefully investigated in order to achieve the best performances in terms of sensitivity and unspecific adsorption. |
回复此楼» 猜你喜欢
材料调剂
已经有4人回复
-
面上模板改不了页边距吧？
已经有6人回复
-
307求调剂
已经有6人回复
-
304求调剂
已经有5人回复
-
317一志愿华南理工电气工程求调剂
已经有8人回复
-
272求调剂
已经有3人回复
-
化工专硕348，一志愿985求调剂
已经有6人回复
-
292求调剂
已经有3人回复
-
290求调剂
已经有6人回复
-
295求调剂
已经有5人回复
