| 查看: 343 | 回复: 0 | |||
liushuai3788新虫 (初入文坛)
|
[交流]
【求助/交流】求翻译 An optical PMMA biochip based on fluorescence
|
|
An optical PMMA biochip based on fluorescence anisotropy:Application to C-reactive protein assay An optical platform for point of care testing(POCT)application is developed.The core of the platform is a miniaturised polymethylmetacrylate(PMMA)chip,that consists of two pieces of PMMA that have been custom-machined to produce flow channels,500 m in width and 400 m in height,through which the analysed sample flows.Thanks to the fluorescence anisotropy,a large fraction of the fluorescence emitted by the sensing layer immobilised on the bottom of the PMMA cover travels along the thickness of the PMMA cover itself up to its end-face and it is collected by a plastic optical fibre connected to a photodetector. The potential of the optical chip is investigated with a sandwich assay for the C-reactive protein,one of the markers for inflammatory diseases.The assay covers the working range from 0.1 to 50 mg L?1 with a limit of detection of 0.004 mg L?1.Different combinations of capture and target antibodies were carefully investigated in order to achieve the best performances in terms of sensitivity and unspecific adsorption. |
回复此楼» 猜你喜欢
271求调剂
已经有34人回复
-
327求调剂
已经有10人回复
-
297求调剂
已经有23人回复
-
材料工程281还有调剂机会吗
已经有33人回复
-
各位老师好，求调剂，本科211，一志愿天津大学生物与医药学硕，差两名录取。
已经有4人回复
-
本科郑州大学，一志愿华东师范大学282求调剂
已经有32人回复
-
求调剂
已经有10人回复
-
一志愿211 0703化学 346分求调剂
已经有29人回复
-
化工学硕294分，求导师收留
已经有22人回复
-
327求调剂
已经有17人回复
20