| 查看: 316 | 回复: 0 | |||
liushuai3788新虫 (初入文坛)
|
[交流]
【求助/交流】求翻译 An optical PMMA biochip based on fluorescence
|
|
An optical PMMA biochip based on fluorescence anisotropy:Application to C-reactive protein assay An optical platform for point of care testing(POCT)application is developed.The core of the platform is a miniaturised polymethylmetacrylate(PMMA)chip,that consists of two pieces of PMMA that have been custom-machined to produce flow channels,500 m in width and 400 m in height,through which the analysed sample flows.Thanks to the fluorescence anisotropy,a large fraction of the fluorescence emitted by the sensing layer immobilised on the bottom of the PMMA cover travels along the thickness of the PMMA cover itself up to its end-face and it is collected by a plastic optical fibre connected to a photodetector. The potential of the optical chip is investigated with a sandwich assay for the C-reactive protein,one of the markers for inflammatory diseases.The assay covers the working range from 0.1 to 50 mg L?1 with a limit of detection of 0.004 mg L?1.Different combinations of capture and target antibodies were carefully investigated in order to achieve the best performances in terms of sensitivity and unspecific adsorption. |
回复此楼» 猜你喜欢
基金委咋了？2026年的指南还没有出来？
已经有8人回复
-
拟解决的关键科学问题还要不要写
已经有4人回复
-
基金申报
已经有5人回复
-
国自然申请面上模板最新2026版出了吗？
已经有17人回复
-
纳米粒子粒径的测量
已经有8人回复
-
疑惑？
已经有5人回复
-
计算机、0854电子信息（085401-058412）调剂
已经有5人回复
-
Materials Today Chemistry审稿周期
已经有5人回复
-
溴的反应液脱色
已经有7人回复
-
推荐一本书
已经有12人回复