Znn3bq.jpeg
ÉÇÍ·´óѧº£Ñó¿ÆÑ§½ÓÊܵ÷¼Á
²é¿´: 1546  |  »Ø¸´: 4

xiangjinle203

Ìú¸Ëľ³æ (ÖøÃûдÊÖ)

[½»Á÷] ¡¾ÇóÖú/½»Á÷¡¿Ñõ»¯Ð͸¨Ã¸IÈÜÒºµÄ±£´æ ÒÑÓÐ4È˲ÎÓë

Ñõ»¯Ð͸¨Ã¸I(NAD+)Åä³ÉÈÜҺʱÓÐÌØÊâÐèҪעÒâµÄÎÊÌâÂð£¿ÅäÖÆ³ÉÈÜÒººóÊDz»ÊǺܲ»Îȶ¨£¿Ôõô±£´æ£¿ÓùýµÄÅóÓѸæËßÏ£¡Ð»Ð»

[ Last edited by xiangjinle203 on 2010-5-4 at 17:57 ]
»Ø¸´´ËÂ¥

» ²ÂÄãϲ»¶

» ±¾Ö÷ÌâÏà¹Ø¼ÛÖµÌùÍÆ¼ö£¬¶ÔÄúͬÑùÓаïÖú:

ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

shwang007

ľ³æ (ÕýʽдÊÖ)

¡ï
Сľ³æ(½ð±Ò+0.5):¸ø¸öºì°ü£¬Ð»Ð»»ØÌû½»Á÷
-30¶È±£´æ£¬´ó¸Å7d£¬²»¹ý£¬×îºÃÓöàÉÙÈܽâ¶àÉÙ
2Â¥2010-05-04 18:27:34
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

HarveyWang

¾èÖú¹ó±ö (ÖªÃû×÷¼Ò)

º£ÍâÐÐÕß

¡ï ¡ï ¡ï ¡ï
Сľ³æ(½ð±Ò+0.5):¸ø¸öºì°ü£¬Ð»Ð»»ØÌû½»Á÷
plantaqp(½ð±Ò+3):»¶Ó­½»Á÷ 2010-05-05 03:31:57
ÒýÓûØÌû:
Originally posted by xiangjinle203 at 2010-05-04 17:52:47:
Ñõ»¯Ð͸¨Ã¸I(NAD+)Åä³ÉÈÜҺʱÓÐÌØÊâÐèҪעÒâµÄÎÊÌâÂð£¿ÅäÖÆ³ÉÈÜÒººóÊDz»ÊǺܲ»Îȶ¨£¿Ôõô±£´æ£¿ÓùýµÄÅóÓѸæËßÏ£¡Ð»Ð»

[ Last edited by xiangjinle203 on 2010-5-4 at 17:57 ]

NAD+»¹ÊDZȽÏÎȶ¨µÄ£¬ÎÒÓÃË«ÕôË®Åä³É5»ò10mMµÄĸҺ£¬1.5 mL С¹Ü·Ö×°£¬-20¡æ±£´æ£¬°ëÄêûÓÐÎÊÌâµÄ¡£

µ«ÊÇNADHǧÍò²»ÄÜÓÃË«ÕôË®ÅäÖã¬NADHÏÔËáÐÔ£¬ÓÃË«ÕôË®ÅäÖÃA340nmÏûʧºÜ¿ì£¬¼´Ê¹ÔÚ-20¡æ±£´æ¡£

Ò»°ã¿ÉÒÔÓà 10 mMµÄ Tris-HCl  pH 8.5-9.0 ±£´æ¡£
»òÓÃNaHCO3  10-20 mMÈÜÒº±£´æ¡£
ÐèÒª¼îÐÔÌõ¼þϲÅÎȶ¨£¬×îºÃ²»ÒªÓÃÁ×ËáÑÎÈÜÒº±£´æ¡£
Ũ¶ÈÒ»°ãΪ4-5 mM£¬ÐèҪʵ¼Ê²â¶¨A340nmÀ´¾ö¶¨ÆäÕæÊµµÄŨ¶È£¬ÒòΪNADHµÄ´¿¶ÈÔÚ80-90%×óÓÒ¡£ÆäÏû¹âϵÊýΪ0.00622(A340nm) --> 1 uM
¡¾ÎÒÒ»Ö±ÈÏΪ×öÍÁ·ËºÜÐԸкܻµ£¬ºÜÖ±½Ó£¬¿ÉÒÔ´óÉùº°£ºJCMMÎÒ°®Ä㣡ËùÒÔ£¬ÎÒ×Ô´Ó²©Ê¿±ÏÒµºó£¬¾ÍÒ»Ö±ÔÚѰÕÒºÚµÀ´ó¸çÒ»ÆðÈ¥ÇÀÇ®¡¢ÇÀÁ¸¡¢ÇÀµØÅÌ¡¿
3Â¥2010-05-04 21:42:44
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

xiangjinle203

Ìú¸Ëľ³æ (ÖøÃûдÊÖ)

[quote]Originally posted by HarveyWang at 2010-05-04 21:42:44:


NAD+»¹ÊDZȽÏÎȶ¨µÄ£¬ÎÒÓÃË«ÕôË®Åä³É5»ò10mMµÄĸҺ£¬1.5 mL С¹Ü·Ö×°£¬-20¡æ±£´æ£¬°ëÄêûÓÐÎÊÌâµÄ¡£

µ«ÊÇNADHǧÍò²»ÄÜÓÃË«ÕôË®ÅäÖã¬NADHÏÔËáÐÔ£¬ÓÃË«ÕôË®ÅäÖÃA340nmÏûʧºÜ¿ì£¬¼´Ê¹ÔÚ-20¡æ±£´æ¡£

Ò»°ã¿É ... [/quote



·Ç³£¸ÐлÄãµÄ»Ø¸´£¡£¡
4Â¥2010-05-04 22:26:04
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

99ÎÏÅ£99

гæ (СÓÐÃûÆø)

¡ï
Сľ³æ: ½ð±Ò+0.5, ¸ø¸öºì°ü£¬Ð»Ð»»ØÌû
ÒýÓûØÌû:
3Â¥: Originally posted by HarveyWang at 2010-05-04 21:42:44
NAD+»¹ÊDZȽÏÎȶ¨µÄ£¬ÎÒÓÃË«ÕôË®Åä³É5»ò10mMµÄĸҺ£¬1.5 mL С¹Ü·Ö×°£¬-20¡æ±£´æ£¬°ëÄêûÓÐÎÊÌâµÄ¡£

µ«ÊÇNADHǧÍò²»ÄÜÓÃË«ÕôË®ÅäÖã¬NADHÏÔËáÐÔ£¬ÓÃË«ÕôË®ÅäÖÃA340nmÏûʧºÜ¿ì£¬¼´Ê¹ÔÚ-20¡æ±£´æ¡£

Ò»°ã¿ÉÒÔ ...

ÄãºÃ£¬NADHÔÚ¼îÐÔÌõ¼þÏÂÎȶ¨£¬±¾ÉíÏÔËáÐÔÒ²²»»áºÍ¼î·´Ó¦Âð£¬ËüÔÚË®Öв»Îȶ¨ÊÇÓÖ·Ö½â³ÉNADÁËÂð£¬Ð»Ð»£¬ÇóÖ¸½Ì
5Â¥2016-03-15 15:46:06
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
Ïà¹Ø°æ¿éÌø×ª ÎÒÒª¶©ÔÄÂ¥Ö÷ xiangjinle203 µÄÖ÷Ìâ¸üÐÂ
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼ÑÐ] ÇóÖúµ÷¼Á£¬¿çµ÷ +14 XÊ®¸¦´çY 2026-04-11 15/750 2026-04-14 00:36 by Íõ¬Bè±
[¿¼ÑÐ] 085600²ÄÁÏÓ뻯¹¤£¬Çóµ÷¼Á +14 won_qii 2026-04-07 14/700 2026-04-13 22:21 by pies112
[¿¼ÑÐ] Çóµ÷¼Á +14 Ñ©·ê¶¬ 2026-04-10 14/700 2026-04-13 21:38 by gretl
[¿¼ÑÐ] ÉúÎïÒ½Ò©µ÷¼Á£üSCIÖпÆÔºÈýÇøÒ»×÷+¶àÏî¿ÆÑгɹû +9 likangxing 2026-04-07 12/600 2026-04-13 21:35 by áÇá¼Ò©Í¯
[¿¼ÑÐ] 290Çóµ÷¼Á +16 luoziheng 2026-04-10 16/800 2026-04-13 20:34 by biomen
[¿¼ÑÐ] Ò»Ö¾Ô¸211 0703»¯Ñ§ 346·ÖÇóµ÷¼Á +26 ÍÁ¶¹er? 2026-04-09 29/1450 2026-04-13 15:15 by ¶À×íÃι³Ç
[¿¼ÑÐ] 346·Ö£¬¹¤¿Æ0854Çóµ÷¼Á£¬×¨Ë¶ +6 moser233 2026-04-12 7/350 2026-04-12 22:11 by fqwang
[¿¼ÑÐ] µç×ÓÐÅÏ¢270Çóµ÷¼Á +18 terminal469 2026-04-07 18/900 2026-04-12 16:23 by ajpv·çÀ×
[¿¼ÑÐ] µçÆø×¨Ë¶320Çóµ÷¼Á +6 СÂé×Ó111 2026-04-10 6/300 2026-04-12 10:54 by lemon6009
[¿¼ÑÐ] 307Çóµ÷¼Á +10 tzq94092 2026-04-10 10/500 2026-04-12 08:18 by wise999
[¿¼ÑÐ] Çóµ÷¼Á +11 ôæôæÒ»ÊéÉú 2026-04-09 11/550 2026-04-11 19:57 by ÄæË®³Ë·ç
[¿¼ÑÐ] Ò»Ö¾Ô¸985»úеѧ˶380Çóµ÷¼Á +5 ¹Ø¹ØöÂð¯10 2026-04-11 5/250 2026-04-11 10:10 by ÖªÄî¡£A
[¿¼ÑÐ] 085601³õÊÔ330·ÖÕÒµ÷¼Á +10 Á÷ÐÄÄ̻ưül 2026-04-09 10/500 2026-04-10 08:14 by Sammy2
[¿¼ÑÐ] 070300»¯Ñ§ Çóµ÷¼Á +13 73372112 2026-04-08 13/650 2026-04-09 20:22 by maddjdld
[¿¼ÑÐ] ¿¼Ñе÷¼Á-²ÄÁÏÀà-284 +28 Ïë»»ÊÖ»ú²»Ïë½âÊ 2026-04-08 28/1400 2026-04-09 20:08 by µ¹Êý321?
[¿¼ÑÐ] 353Çóµ÷¼Á +8 Çç¿ÕÍòÀïair 2026-04-07 8/400 2026-04-09 00:18 by GouQ
[¿¼ÑÐ] µç×ÓÐÅÏ¢346 +4 zuoshaodian 2026-04-08 4/200 2026-04-08 11:54 by zzucheup
[¿¼ÑÐ] 323Çóµ÷¼Á +3 ÁÖzlu 2026-04-07 4/200 2026-04-07 23:21 by lbsjt
[¿¼ÑÐ] 11408 325·Ö +3 jgtxuxgkx 2026-04-07 3/150 2026-04-07 23:10 by lbsjt
[¿¼ÑÐ] ר˶085403£¬291·Ö£¬ÓÐÁ½Æª×¨Àû£¬Ò»¹úÒ»½± +3 ¹þ¼ªßä¹þ¼ªßä 2026-04-07 3/150 2026-04-07 18:21 by À¶ÔÆË¼Óê
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û