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To determine the cell envelope proteins of Borrelia burgdorferi recognized by immune sera of patients with late Lyme disease,we developed a Borrelia microarray containing proteins encoded by 90 cell envelope genes and their homologs described in the annotated genomic sequence of B.burgdorferi,strain B31.The protein microarray was used to profile the humoral immune response using sera from 13 patients with late Lyme disease and four normal controls.Although there was considerable heteroge-neity in the individual sera responses,25 of the cell envelope proteins were recognized by seven or more samples.Sera from non-infected individuals lacked reactivity against any of the proteins on the array. Among the most antigenic envelope proteins,BLAST search revealed little sequence homology to known microbial proteins from other species.The proteins that were highly seropositive included several members of the Erp gene families,BBA24(decorin binding protein A(DbpA))and members of the Borrelia gene family Pfam113 that code for the Mlp lipoprotein gene family.Several novel,uncharac-terized B.burgdorferi antigens identified in this study were BBA14,BBG23,BB0108,BB0442 and BBQ03. The accurate diagnosis of Lyme disease depends on correlating objective clinical abnormalities with serological evidence of exposure to B.burgdorferi.A protein array of the envelope proteins of Borrelia burgdorferi may be very useful in specifically identifying patients with Lyme disease.This approach could contribute to a more rapid discovery of antigens not expressed in vitro that may be useful for the development of vaccine and diagnostics. |
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liushuai3788(金币+10): 2010-05-01 11:14
amisking(金币+5): 2010-05-01 12:39
liushuai3788(金币+10): 2010-05-01 11:14
amisking(金币+5): 2010-05-01 12:39
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为了确定病人免疫血清所公认的晚期莱姆病莱姆病螺旋体细胞膜蛋白质，我们制定了一个莱姆芯片包含了90细胞中的基因和信封注明的B.burgdorferi，应变B31座基因序列的同源基因编码的蛋白质的描述。该蛋白芯片，用于配置文件的体液免疫反应使用来自13个血清 莱姆病晚期和4例正常controls.Although患者有相当大的异构- neity在个别血清反应，细胞的膜蛋白有25人承认，7个或更多的非受感染的个人samples.Sera缺乏对任何反应的蛋白质阵列上。 其中最信封蛋白抗原，BLAST搜索发现很少从其他已知的序列同源性的蛋白质，具有高度species.The阳性微生物蛋白包括几个 的ERP基因家族成员，BBA24（核心蛋白聚糖结合蛋白A（DbpA））和莱姆基因家族Pfam113，对于总纲发展蓝图脂蛋白基因family.Several新颖，uncharac，电导率对其B.burgdorferi代码成员抗原这项研究确定出了BBA14 ，BBG23，BB0108，BB0442和BBQ03。 莱姆病的准确诊断有赖于关联与接触B.burgdorferi.A对莱姆病的血清学包膜蛋白的蛋白质阵列客观证据临床异常可能是非常具体的方式确定与莱姆disease.This患者可能有用 有助于更快速的抗原的发现，在体外表达，可能是对疫苗和诊断的发展有益。 |
2楼2010-05-01 10:58:49