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The diet was offered to the mites through a parafilm membrane. A piece of parafilm, sterilized by immersion in 70% ethanol, was stretched across the top of a 3 cm in diameter and 2 cm in height sterile Petri dish. One ml of the diet was applied to the membrane and a second piece of sterile parafilm was stretched over the diet and sealed against the side of the Petri dish. Care was taken that no air bubbles were trapped underneath the second membrane. A steep ridge of the Petri dish (¡°diet container¡±)was surrounded by a piece of wet tissue paper and the diet containers were placed in another Petri dish (9 cm in diameter and 1.5 cm in height) filled with water to confine the mites on the diet area. Twenty newly emerged protonymphs of P. citri were randomly selected from the stock culture and placed onto each of 10 diet containers.The survivors and their stages were recorded every day on all diet containers until the mites reached deutonymphs. |
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