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suger_fan

木虫 (正式写手)

虫坛啄木鸟

[交流] 请帮忙翻译一段关于昆虫饲养方面的英文

The diet was offered to the mites through a parafilm membrane. A piece of parafilm, sterilized by immersion in 70% ethanol, was stretched across the top of a
3 cm in diameter and 2 cm in height sterile Petri dish. One ml of the diet was applied to the membrane and a second piece of sterile parafilm was stretched over the diet and sealed against the side of the Petri dish. Care was taken that no air bubbles were trapped underneath the second membrane. A steep ridge of the Petri dish (“diet container”)was surrounded by a piece of wet tissue paper and
the diet containers were placed in another Petri dish (9 cm in diameter and 1.5 cm in height) filled with water to confine the mites on the diet area. Twenty newly emerged protonymphs of P. citri were randomly selected from the stock culture and placed onto each of 10 diet containers.The survivors and their stages were recorded every day on all diet containers until the mites reached deutonymphs.
宠辱不惊,笑看堂前花开花落;去留无意,漫随天外云卷云舒。
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apple200507

金虫 (正式写手)

suger_fan(金币+20): 2010-04-15 08:59
通过石蜡膜给mite喂食,将石蜡膜用70%的乙醇消毒,然后展开在直径3cm,高2cm的消毒的皮氏培养皿的表面。在膜表面放上1ml的食物,然后在该膜表面再铺一层消毒的石蜡膜,将其展开并让它的边缘密封住培养皿。注意第二层膜下不能有气泡。 用湿的薄纸把该培养皿(喂食容器R:3cm,H:2cm)的外壁包裹住,然后把所有这些用于喂食的培养皿放在另一个装有水的皮氏培养皿中(直径:9cm,高1.5cm),已限制mites就在喂食区。对于每个喂食培养皿(共10个),从储备培养中随机选取二十只新出现的P.citri 第一若虫,放于其上。喂食所有喂食培养皿上的存活者及其发展阶段都被记录直到所有的mite都达到第二若虫阶段。
很多专业词汇翻译得生硬,不过大体意思应该是这样,不知道帮得到楼主不。。。

[ Last edited by apple200507 on 2010-4-13 at 17:54 ]
2楼2010-04-13 17:53:27
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shirongjiu

金虫 (正式写手)

suger_fan(金币+30, 翻译EPI+1): 2010-04-15 08:57
通过石蜡膜给螨虫喂食。选一张浸过70%乙醇消毒的石蜡膜,然后展开在直径3cm,高2cm的消毒的培养皿表面。在膜表面放上1ml的食物,然后在该膜表面再铺一层消毒的石蜡膜,将其展开并让它的边缘密封住培养皿。注意第二层膜下不能有气泡。 用湿的薄纸把该培养皿(喂食容器R:3cm,H:2cm)的外壁包裹住,然后把所有这些用于喂食的培养皿放在另一个装有水的培养皿中(直径:9cm,高1.5cm),以限制螨虫就在该喂食区。从种子培养皿中随机选取二十只第一若螨(幼螨)放置在每一个喂食培养皿(共10个)上,每天记录喂食培养皿上的存活者及其发育阶段,直至第一若螨达到第二若螨阶段。
3楼2010-04-14 10:58:40
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