| 查看: 301 | 回复: 4 | |||
| 当前主题已经存档。 | |||
| 本帖产生 1 个 博学EPI ，点击这里进行查看 | |||
472514568至尊木虫 (职业作家)
小木虫议政大臣兼协办大学士
|
[交流]
求文献一篇 有链接
|
||
|
【作者(必填)】 Deng Y, Bi HC, Zhao LZ, Wang XD, Chen J, Ou ZM, Ding L, Xu LJ, Guan S, Chen X, Zhou SF, Huang M. 【文题(必填)】 Induction of cytochrome P450 3A by the Ginkgo biloba extract and bilobalides in human and rat primary hepatocytes. 【期刊名,年份,卷(期),起止页码(必填)】 Drug Metab Lett. 2008 Jan;2(1):60-6. 【全文链接或数据库名称(选填)】 http://www.ncbi.nlm.nih.gov/pubmed/19356072 |
回复此楼» 猜你喜欢
281求调剂（0805）
已经有8人回复
-
环境领域全国重点实验室招收博士1-2名
已经有3人回复
-
材料专硕306英一数二
已经有10人回复
-
301求调剂
已经有6人回复
-
一志愿天津大学化学工艺专业（081702）315分求调剂
已经有7人回复
-
302求调剂
已经有6人回复
-
26博士申请
已经有3人回复
-
268求调剂
已经有3人回复
-
311求调剂
已经有10人回复
-
被我言中：新模板不强调格式了，假专家开始管格式了
已经有4人回复
luoxianyang
银虫 (正式写手)
- 博学EPI: 3
- 应助: 0 (幼儿园)
- 金币: 335.3
- 红花: 1
- 帖子: 635
- 在线: 68小时
- 虫号: 921566
- 注册: 2009-12-06
- 性别: GG
- 专业: 药物代谢与药物动力学
472514568(金币+1, 博学EPI+1):虽然没有找到全文 但还是很感谢你 2010-04-12 16:30
|
仅找到摘要，不好意思。 Abstract: Ginkgo biloba is one of the most popular herbal medicines in the world, due to its purported pharmacological effects, including memory-enhancing, cognition-improving, and antiplatelet effects. The study aimed to investigate the activity and expression of cytochrome P450 (CYP) 3A in human and rat primary hepatocytes treated with standardized G. biloba extract (100, 500, and 2500 ng/ml) for 72 hr, and to measure the protein expression of CYP3A in human and rat primary hepatocytes treated with bilobalide (2, 10, and 50 ng/ml) and ginkgolides B (2, 10, and 50 ng/ml). The activity of CYP3A was measured by the quantification of dehydronifedipine formation using a validated tandem liquid chromatography mass spectrometry (LC/MS/MS) method. The levels of mRNA and protein of CYP3A were determined by reverse transcription-polymerase chain reaction (RT-PCR) and Western-blotting analysis, respectively. The G. biloba extract at 100-2,500 ng/ml significantly induced the activity, protein and mRNA expression of CYP3A in a dose-dependent manner in human and rat primary hepatocytes. Bilobalide at 2-50 ng/ml significantly increased CYP3A protein expression in a dose-dependent manner in human and rat primary hepatocytes. However, ginkgolide B did not affect CYP3A protein expression in vitro. The results indicate that G. biloba extract pretreatment significantly induced the expression of CYP3A protein and mRNA and increased CYP3A activity, and there was no significant species difference between human and rat. G. biloba may cause potential interactions with substrate drugs of CYP3A. Bilobalide might play a key role in the enzyme- inducing effects of G. biloba extract. Further study is needed to identify the substances in GBE that induce CYPs in vivo, and elucidate the molecular mechanism of CYP3A induction by GBE and bilobalides. Keywords: Ginkgo biloba extract; bilobalide; ginkgolide B; cytochrome P450; CYP3A; induction; human and rat primary hepatocytes Document Type: Research article DOI: 10.2174/187231208783478489 |
2楼2010-04-11 16:09:36
jlliu2000
至尊木虫 (著名写手)
- 应助: 0 (幼儿园)
- 金币: 12042.1
- 散金: 1020
- 红花: 4
- 帖子: 1897
- 在线: 233.3小时
- 虫号: 774003
- 注册: 2009-05-19
- 专业: 胶体与界面化学
3楼2010-04-11 16:12:52
cedefe
木虫 (正式写手)
- 博学EPI: 5
- 应助: 2 (幼儿园)
- 金币: 4989.9
- 散金: 85
- 红花: 5
- 帖子: 723
- 在线: 64.2小时
- 虫号: 779233
- 注册: 2009-05-25
- 专业: 有机分子功能材料化学
4楼2010-04-11 18:33:33
alwaysmart
铁杆木虫 (职业作家)
飞天猫
- 博学EPI: 1
- 应助: 3 (幼儿园)
- 金币: 9795.5
- 沙发: 1
- 帖子: 3030
- 在线: 102.8小时
- 虫号: 573402
- 注册: 2008-06-14
- 性别: GG
- 专业: 能源化工
|
注册这个，难找文献他们帮你下 广东省科技图书馆虚拟参考咨询与原文传递平台是一个基于用户互助和积分激励机制的免费原文传递和虚拟参考咨询平台，为注册用户提供免费的中外文期刊、会议论文、图书章节等文献传递服务。广东省科技图书馆具有丰富的中外文科技文献，并且有一批热情的原文传递专家为平台用户服务；用户们通过该平台在获得别人帮助的同时，也可以通过帮助其他人获得积分奖励。该平台响应速度快，服务周到。赶快去注册吧！ 您点击这个链接 http://www.dlrp.proxy.stlib.cn/d ... ?inviter=alwaysmart 并成功注册，我就能得到该系统奖励的积分；注册后，您同样也可以向其他好友发邀请哦！ |
5楼2010-04-11 18:53:17
