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| After cell enrichment, cells were released and enumerated using benchtop real-time quantitative polymerase chain reaction (PCR), targeting genes which effectively discriminated the O157:H7 serotype from other nonpathogenic bacteria. The recovery of target cells from water samples was determined to be ∼72%, and the limit-of-detection was found to be 6 colony forming units (cfu) using the slt1 gene as a reporter. |
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