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lzhwin木虫 (正式写手)
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[交流]
【求助】新手提问：关于SUPELCOSIL ABZ+Plus色谱柱
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在一篇方法学文章里看到用LC/MS/MS方法定量植物鞘脂。用到了标题里的那根柱子。请问各位大虾有人用过吗？能不能介绍一下这个柱子有什么特别的？为什么那么贵（sigma价格10000大元）？附上sigma里的一段介绍 链接：http://www.sigmaaldrich.com/cata ... RAND_KEY&F=SPEC Features and Benefits • High efficiency for polar, nonpolar, and charged analytes • Symmetric peaks for the most reactive compounds • Unique selectivity for polar and charged compounds General description SUPELCOSIL ABZ+Plus columns offer both high deactivation and unique selectivity. Deactivated silica particles of very narrow particle size distribution ensure high efficiency with low back pressure. After bonding and endcapping reactions, the ABZ+Plus phase effectively shields unreacted silanol groups on the silica, preventing them from interacting with most analytes, and provides symmetric peaks regardless of an analyte′s functionality. The phase also allows you to use low ionic strength buffers without having to add an ion-suppressing modifier. ABZ+Plus enables you to use simple mobile phases when analyzing the most difficult compounds; acids, strongly basic compounds, and zwitterions. suitable for L60 per USP 问题：1、是不是只能用来做反相HPLC？ 2、这个柱子分离的原理是根据什么？是脂肪酸链的长度或饱和度还是极性头部的特性（比如是否带电，疏水性等等）？ 3、有没有人用过这个柱子？可否用QQ或者邮件交流一下？ 我的QQ173293769 [ Last edited by lzhwin on 2010-3-30 at 14:12 ] |
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2楼2010-03-30 17:37:32