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重金悬赏翻译一段微生物方面英文文献
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Serratia marcescens Simon Swift-1 (SS-1) was used to produce a prodigiosin-like pigment, undecylprodigiosin(UP), known to have antitumor activities and potential as an anticancer drug.Modified media containing components of Luria–Bertani (LB) broth and selected amino acids were used to improve UP production from S. marcescens SS-1. Optimal culture conditions (e.g.,temperature, pH, agitation rate) for UP production were also identified. It was found that S.marcescens SS-1 was able to produce 690mg l–1 of UP when it was grown with 5 g l–1 yeast extract alone (YE medium) under the optimal culture conditions of 30°C, 200 rpm, and pH 8. The UP production of 690mg l–1 is nearly 23-fold of that obtained from original LB medium. Addition of amino acids containing pyrrole-like structures further enhanced UP production. Nearly 2 and 1.4 g l–1 of UP was produced when the SS-1 strain was cultivated with YE medium supplemented with proline and histidine (5 g l–1), respectively. Moreover, the addition of aspartic acid (5 g l–1) also resulted in a high UP production of 1.4 g l–1. Optimal dosages of the three amino acids were subsequently determined and the highest UP production (2.5 g l–1) was achieved with the addition of 10 g l–1 of proline. This suggests that the supplementation of amino acids related to the formation of a UP precursor (e.g., pyrrolylpyrromethene) could enhance UP production by the SS-1 [ Last edited by phyweiw on 2010-3-4 at 21:22 ] |
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xiaoqihu
铁杆木虫 (著名写手)
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【答案】应助回帖
duweihappy(金币+10):谢谢！ 2010-03-05 16:11
duweihappy(金币+1, 翻译EPI+1):太差了 2010-04-07 20:23
duweihappy(金币+1):buhao 2010-05-05 11:01:41
duweihappy(金币+1, 翻译EPI+1):太差了 2010-04-07 20:23
duweihappy(金币+1):buhao 2010-05-05 11:01:41
| Serratia marcescens Simon Swift-1 (SS-1)被用做产生灵菌红素状的色素, 十一烷基灵菌红素（UP）具有抗肿瘤活性，是潜在的抗癌症药物。 经过修饰的含有LB培养基和经过挑选的氨基酸的介质被用作改进提高从SS-1产生UP的产量。优化的UP产生培养条件（例如温度，酸碱度，搅拌速率）同样也给出了。研究表明，SS-1在只有5 g /L的酵母提取物(YE medium)的优化培养条件下（30°C, 200 rpm, and pH 8.）能产生690 mg/L 的UP。UP的690mg的产量几乎和23折叠的最初LB产生的接近。额外的含有氨基酸的吡咯状结构能进一步提高UP的产量。当SS-1菌株被培养在有吡咯YE介质和组氨酸YE介质的UP产量分别为2g和1.4g。而且，加入天门冬氨酸同样也能提高UP的产量得到1.4g/L。随后对三个氨基酸的用量进行了优化，在添加了10g/L的吡咯条件下最终得到最高2.5g/L的UP产量。这个结论说明了与产生UP前体（例如pyrrolylpyrromethene）相关的氨基酸的辅助能提高从SS-1产生UP的产量 |
2楼2010-03-04 22:39:53